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![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Nuclear extract (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JMJD2A antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Western-Blot-NB110-40585-img0007.jpg "Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Nuclear extract (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JMJD2A antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.")
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Lamins A/C-53BP1 interaction is dependent on the 53BP1 Tudor domain. U2OS/GFP-lamin A cells were subjected to IR (10 Gy) and allowed to recover for 1 h. Cell extracts were then subjected to immunoprecipitation using GFP-Trap beads, and bound complexes were then analyzed by immunoblotting using GFP, LAP2alpha and JMJD2A antibodies. WCE represents 1% input. Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1111/acel.12258), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Western-Blot-NB110-40585-img0006.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - Detection of human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A by western blot of immunoprecipitates. Samples: Nuclear Extract (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody NB110-40585 used for IP at 6 ug per reaction. Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A was also immunoprecipitated by rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody NB110-40584. For blotting immunoprecipitated Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A, NB110-40585 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Immunoprecipitation-NB110-40585-img0005.jpg)
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - KDM4A interacts with the mTORC1/2 complex.(a) Relative mRNA levels of negative & positive regulators of the PTEN/AKT/mTOR pathway following KDM4A depletion. Quantifications of mRNAs by RT–qPCR were normalized against beta -actin (ActB) mRNA. Asterisks denote a statistical difference between siKDM4A-treated cells & siGFP control cells, two-sided t-test P<0.05 (graph represents two independent experiences). Error bars represent standard deviation. (b) Co-immunoprecipitation of endogenous mTORC1/2 complex members with Flag-KDM4A in 293T transfected cells. (c) Comparison of mTORC1/2-associated proteins with Flag-tagged mTOR or KDM4A. The 293T cells were transfected with either Flag-eYFP, Flag-KDM4A or Flag-mTOR, & protein lysates were subjected to anti-Flag immunoprecipitation. (d) Co-immunoprecipitation of Flag-KDM4A & HA-DEPTOR in 293T cells. (e) Endogenous KDM4A co-immunoprecipitates with Flag-DEPTOR. (f) DEPTOR PDZ domain associates with endogenous KDM4A. Flag immunoprecipitation of flag-tagged full length or fragments of DEPTOR. The samples were not sonicated in this experiment to confirm that the interaction is independent of nucleus disruption. (g) Endogenous KDM4A & DEPTOR associate in 293E cells. (h) Endogenous KDM4A & DEPTOR co-immunoprecipitate in NHA-hTERT cells. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms12700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40585_rabbit-polyclonal-lysine-k-specific-demethylase-4a-kdm4a-jmjd2a-antibody-310202416235537.jpg)
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - KDM4A interacts with the mTORC1/2 complex.(a) Relative mRNA levels of negative & positive regulators of the PTEN/AKT/mTOR pathway following KDM4A depletion. Quantifications of mRNAs by RT–qPCR were normalized against beta -actin (ActB) mRNA. Asterisks denote a statistical difference between siKDM4A-treated cells & siGFP control cells, two-sided t-test P<0.05 (graph represents two independent experiences). Error bars represent standard deviation. (b) Co-immunoprecipitation of endogenous mTORC1/2 complex members with Flag-KDM4A in 293T transfected cells. (c) Comparison of mTORC1/2-associated proteins with Flag-tagged mTOR or KDM4A. The 293T cells were transfected with either Flag-eYFP, Flag-KDM4A or Flag-mTOR, & protein lysates were subjected to anti-Flag immunoprecipitation. (d) Co-immunoprecipitation of Flag-KDM4A & HA-DEPTOR in 293T cells. (e) Endogenous KDM4A co-immunoprecipitates with Flag-DEPTOR. (f) DEPTOR PDZ domain associates with endogenous KDM4A. Flag immunoprecipitation of flag-tagged full length or fragments of DEPTOR. The samples were not sonicated in this experiment to confirm that the interaction is independent of nucleus disruption. (g) Endogenous KDM4A & DEPTOR associate in 293E cells. (h) Endogenous KDM4A & DEPTOR co-immunoprecipitate in NHA-hTERT cells. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms12700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40585_rabbit-polyclonal-lysine-k-specific-demethylase-4a-kdm4a-jmjd2a-antibody-310202416235544.jpg)
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NB110-40585] - KDM4A interacts with the mTORC1/2 complex.(a) Relative mRNA levels of negative & positive regulators of the PTEN/AKT/mTOR pathway following KDM4A depletion. Quantifications of mRNAs by RT–qPCR were normalized against beta -actin (ActB) mRNA. Asterisks denote a statistical difference between siKDM4A-treated cells & siGFP control cells, two-sided t-test P<0.05 (graph represents two independent experiences). Error bars represent standard deviation. (b) Co-immunoprecipitation of endogenous mTORC1/2 complex members with Flag-KDM4A in 293T transfected cells. (c) Comparison of mTORC1/2-associated proteins with Flag-tagged mTOR or KDM4A. The 293T cells were transfected with either Flag-eYFP, Flag-KDM4A or Flag-mTOR, & protein lysates were subjected to anti-Flag immunoprecipitation. (d) Co-immunoprecipitation of Flag-KDM4A & HA-DEPTOR in 293T cells. (e) Endogenous KDM4A co-immunoprecipitates with Flag-DEPTOR. (f) DEPTOR PDZ domain associates with endogenous KDM4A. Flag immunoprecipitation of flag-tagged full length or fragments of DEPTOR. The samples were not sonicated in this experiment to confirm that the interaction is independent of nucleus disruption. (g) Endogenous KDM4A & DEPTOR associate in 293E cells. (h) Endogenous KDM4A & DEPTOR co-immunoprecipitate in NHA-hTERT cells. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/ncomms12700), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-40585_rabbit-polyclonal-lysine-k-specific-demethylase-4a-kdm4a-jmjd2a-antibody-310202416235523.jpg)
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Analysis of JMJDA in HeLa nuclear extracts.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Western-Blot-NBP1-49602-img0006.jpg "Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Analysis of JMJDA in HeLa nuclear extracts.")
![Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei (Blue) were counterstained with Dapi (blue).](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-49602-img0004.jpg)
![Immunohistochemistry: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Staining of JMJD2A in paraffin embedded mouse pancreas.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Immunohistochemistry-NBP1-49602-img0005.jpg)
![Simple Western: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-49602] - Simple Western lane view shows a specific band for JMJD2A in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Simple-Western-NBP1-49602-img0007.jpg)
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-87855] - Analysis in human cell line HEK 293.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Western-Blot-NBP1-87855-img0009.jpg "Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-87855] - Analysis in human cell line HEK 293.")
![Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-87855] - Staining of human cell line U-2 OS shows positivity in nucleoli.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87855-img0007.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-87855] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Immunohistochemistry-Paraffin-NBP1-87855-img0014.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-87855] - Staining of human cerebellum shows strong positivity in nucleoli in Purkinje cells.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Immunohistochemistry-Paraffin-NBP1-87855-img0010.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-87855] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Immunohistochemistry-Paraffin-NBP1-87855-img0011.jpg)
![Immunohistochemistry-Paraffin: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP1-87855] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Immunohistochemistry-Paraffin-NBP1-87855-img0013.jpg)
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (PSH03-67) [NBP3-32493] - Western blot analysis of Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A on different lysates with Rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody (NBP3-32493) at 1/2,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32493_rabbit-lysine-k-specific-demethylase-4a-kdm4a-jmjd2a-mab-278202415212499.jpg "Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (PSH03-67) [NBP3-32493] - Western blot analysis of Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A on different lysates with Rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody (NBP3-32493) at 1/2,000 dilution.")
![Immunohistochemistry: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (PSH03-67) [NBP3-32493] - Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody (NBP3-32493) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32493) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32493_rabbit-lysine-k-specific-demethylase-4a-kdm4a-jmjd2a-mab-298202414262617.jpg)
![Immunocytochemistry/ Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (PSH03-67) [NBP3-32493] - Immunocytochemistry analysis of NCCIT cells labeling Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A with Rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody (NBP3-32493) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody (NBP3-32493) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32493_rabbit-lysine-k-specific-demethylase-4a-kdm4a-jmjd2a-mab-29202410234253.jpg)
![Flow Cytometry: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (PSH03-67) [NBP3-32493] - Flow cytometric analysis of NCCIT cells labeling Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32493, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32493_rabbit-lysine-k-specific-demethylase-4a-kdm4a-jmjd2a-mab-29202416453277.jpg)
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Western blot analysis using KDM4A mAb against human KDM4A (AA: 932-1057) recombinant protein. (Expected MW is 40.6 kDa)](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-6E10G4-Western-Blot-NBP2-52499-img0003.jpg "Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Western blot analysis using KDM4A mAb against human KDM4A (AA: 932-1057) recombinant protein. (Expected MW is 40.6 kDa)")
![Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Analysis of HeLa cells using KDM4A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-6E10G4-Immunocytochemistry-Immunofluorescence-NBP2-52499-img0006.jpg)
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Analysis using KDM4A mAb against HEK293 (1) and KDM4A (AA: 932-1057)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-6E10G4-Western-Blot-NBP2-52499-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Analysis of HeLa cells using KDM4A mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-6E10G4-Immunocytochemistry-Immunofluorescence-NBP2-52499-img0005.jpg)
![ELISA: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (6E10G4) [NBP2-52499] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-6E10G4-ELISA-NBP2-52499-img0007.jpg)
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP3-18688] - Figure 2. Western blot analysis using the antibody directed against Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using the antibody against Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size 121 kDa); the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-Western-Blot-NBP3-18688-img0001.jpg "Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP3-18688] - Figure 2. Western blot analysis using the antibody directed against Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Nuclear extracts from HeLa cells (40 ug) were analysed by Western blot using the antibody against Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size 121 kDa); the marker (in kDa) is shown on the left.")
![ELISA: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody [NBP3-18688] - Figure 1. Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A , crude serum and flow through. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:9,000.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-ELISA-NBP3-18688-img0002.jpg)
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (S01-7F2) [NBP3-15055] - Western blot detection of Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A in Hela, A549, HL-60, U2OS, U87-MG cell lysates using Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Rabbit mAb (1:1000 diluted). Observed band size: 150kDa.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-S01-7F2-Western-Blot-NBP3-15055-img0001.jpg "Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (S01-7F2) [NBP3-15055] - Western blot detection of Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A in Hela, A549, HL-60, U2OS, U87-MG cell lysates using Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Rabbit mAb (1:1000 diluted). Observed band size: 150kDa.")
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (JE36-54) [NBP3-32492] - Western blot analysis of Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A on different lysates with Rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody (NBP3-32492) at 1/1,000 dilution. Lane 1: MG-63 cell lysate (20 ug/Lane) Lane 2: Mouse cerebellum tissue lysate (30 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: Rat brain tissue lysate (30 ug/Lane) Predicted band size: 121 kDa Observed band size: 121 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32492) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32492_rabbit-lysine-k-specific-demethylase-4a-kdm4a-jmjd2a-mab-278202415212441.jpg "Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (JE36-54) [NBP3-32492] - Western blot analysis of Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A on different lysates with Rabbit anti-Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A antibody (NBP3-32492) at 1/1,000 dilution. Lane 1: MG-63 cell lysate (20 ug/Lane) Lane 2: Mouse cerebellum tissue lysate (30 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: Rat brain tissue lysate (30 ug/Lane) Predicted band size: 121 kDa Observed band size: 121 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32492) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (9A9G6) [NBP3-16799] - Western blot analysis of extracts of various cell lines, using Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Rabbit mAb (NBP3-16799) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Lysine-K-specific-Demethylase-4A-KDM4A-JMJD2A-Antibody-9A9G6-Western-Blot-NBP3-16799-img0001.jpg "Western Blot: Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Antibody (9A9G6) [NBP3-16799] - Western blot analysis of extracts of various cell lines, using Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A Rabbit mAb (NBP3-16799) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: JMJD2A Overexpression Lysate (Adult Normal) [NBL1-12103] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for JMJD2A.](http://images.novusbio.com/fullsize/JMJD2A-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12103-img0002.jpg "Western Blot: JMJD2A Overexpression Lysate (Adult Normal) [NBL1-12103] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for JMJD2A.")
![ELISA: Human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A ELISA Kit (Colorimetric) [NBP3-40048] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40048_human-lysine-k-specific-demethylase-4a-kdm4a-elisa-kit-211020241538016.png "ELISA: Human Lysine (K)-specific Demethylase 4A/KDM4A/JMJD2A ELISA Kit (Colorimetric) [NBP3-40048] - Standard Curve Reference")