Product Search for HDAC: Novus Biologicals

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299 Results for "HDAC"

NB100-56340

HDAC1 Antibody

(2 Reviews)

(19 Publications)

0.1 mg

NB100-56340

Knockout Validated: HDAC1 Antibody [NB100-56340] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HDAC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 2.0 ug/ml of Rabbit Anti-Human HDAC1 Polyclonal Antibody (Catalog # NB100-56340) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HDAC1 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.

<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NB100-56340] - Analysis of HDAC1 in HEK293 cell lysate with this antibody.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NB100-56340] - Human testis using HDAC1 antibody at 1:250 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.

Simple Western: HDAC1 Antibody [NB100-56340] - Simple Western lane view shows a specific band for HDAC1 in 0.5 mg/ml of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-337

Alt Catalog# IMG-337

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NB100-1669

HDAC3 Antibody

(9 Publications)

0.1 mg

NB100-1669

Western Blot: HDAC3 Antibody [NB100-1669] - Analysis of HDAC3 in HeLa cell lysate with anti-HDAC3 this antibody.

Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NB100-1669] - HDAC3 preferably binds to nuclear Htt with long Qs. HDAC3 associates exclusively with nuclear inclusion bodies. E3 or N3 cells were fixed and stained with anti-HDAC3 antibodies and visualized by Alexa 546 conjugated secondary antibodies. Arrowheads: inclusion bodies with no HDAC3 signals associated. Arrows: HDAC3 signal-associated inclusion bodies. Bar=20 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111277), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: HDAC3 Antibody [NB100-1669] - Analysis human colon cancer using 1:50 conc. of HDAC3 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of HDAC3 was observed. Staining was performed by Histowiz.

Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NB100-1669] - HDAC3 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue). An antibody dilution of 1:10 was used. Image objective 40x.

Simple Western: HDAC3 Antibody [NB100-1669] - Lane view shows a specific band for HDAC3 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

$HDAC3 preferably binds to nuclear Htt with long Qs.A: GST-HDAC3 binds directly to either cytoplasmic or nuclear Htt-ex1. GST pull-down assay of E1, E2, E3 (cytoplasm), and N1, N2, N3 (nuclear) HeLa cell lysates is shown. Pulled-down fraction was analyzed by anti-GFP or GST antibodies. *Non-specific band. B: HDAC3 immunoprecipitates almost exclusively with nuclear Htt-ex1s. E1, E3 (cytoplasm), N1, and N3 (nuclear) HeLa cells were transfected with FLAG-tagged HDAC3 and those lysates were immunoprecipitated with anti-FLAG antibodies immobilized to protein G agarose beads. The pre-IP fraction and the IPed fraction were analyzed using anti-FLAG or anti-GFP antibodies. Molecular weight markers are shown on the left. C: HDAC3 associates exclusively with nuclear inclusion bodies. E3 or N3 cells were fixed and stained with anti-HDAC3 antibodies and visualized by Alexa 546 conjugated secondary antibodies. Arrowheads: inclusion bodies with no HDAC3 signals associated. Arrows: HDAC3 signal-associated inclusion bodies. Bar = 20 um. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0111277), licensed under a CC-BY licence.$

Reactivity:

Hu, Mu, Rt, Ca, Ch, Fe, Pm, Xp

Species Glossary

Reactivity: Hu, Mu, Rt, Ca, Ch, Fe, Pm, Xp

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-308

Alt Catalog# IMG-308

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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NBP2-22152

HDAC5 Antibody - BSA Free

(1 Review)

(5 Publications)

0.1 ml

NBP2-22152

Western Blot: HDAC5 Antibody [NBP2-22152] - LNCaP cells with normal medium and with androgen depletion. Image from verified customer review.

<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HDAC5 Antibody [NBP2-22152] - HDAC5 antibody was tested in A431 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry: HDAC5 Antibody [NBP2-22152] - IHC analysis of HDAC5 in mouse pancreas.

Western Blot: HDAC5 Antibody [NBP2-22152] - WB analysis of HDAC5 in 1. HeLa, 2. Ntera 2, 3. HepG2 and 4. MCF7 cell lysates.

Reactivity:

Hu, Mu, Rt, Pm

Species Glossary

Reactivity: Hu, Mu, Rt, Pm

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22152AF350
Alexa Fluor 405	NBP2-22152AF405
Alexa Fluor 488	NBP2-22152AF488
Alexa Fluor 532	NBP2-22152AF532
Alexa Fluor 594	NBP2-22152AF594
Alexa Fluor 647	NBP2-22152AF647
Alexa Fluor 700	NBP2-22152AF700
Alexa Fluor 750	NBP2-22152AF750
Allophycocyanin	NBP2-22152APC
Biotin	NBP2-22152B
DyLight 350	NBP2-22152UV
DyLight 405	NBP2-22152V
DyLight 488	NBP2-22152G
DyLight 550	NBP2-22152R
DyLight 594	NBP2-22152DL594
DyLight 650	NBP2-22152C
DyLight 680	NBP2-22152FR
DyLight 755	NBP2-22152IR
FITC	NBP2-22152F
HRP	NBP2-22152H
Janelia Fluor 549	NBP2-22152JF549
Janelia Fluor 646	NBP2-22152JF646
PE	NBP2-22152PE
PerCP	NBP2-22152PCP
mFluor Violet 450 SE	NBP2-22152MFV450
mFluor Violet 500 SE	NBP2-22152MFV500
mFluor Violet 610 SE	NBP2-22152MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22152SS

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NB500-126

HDAC3 Antibody - BSA Free

(9 Publications)

0.05 ml

NB500-126

Western Blot: HDAC3 Antibody - BSA Free [NB500-126] - Cell line lysates were separated on SDS-PAGE and probed with 1:5,000 Rabbit Anti-HDAC3. The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. HEK-293T; 2. HeLa; 3. K562; 4. COS-7; 5. CHO; 6. NIH-3T3; 7. Neuro-2a; 8. PC-12; 9. NRK

Immunocytochemistry/Immunofluorescence: HDAC3 Antibody - BSA Free [NB500-126] - HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1:200 Rabbit Anti-HDAC3. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

Immunohistochemistry: HDAC3 Antibody - BSA Free [NB500-126] - Formalin-fixed, paraffin-embedded section of lymph node stained with 1:500 Anti-Histone Deacetylase 3 (HDAC3) antibody produced in Rabbit followed by Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by ExtrAvidin(R)-Peroxidase

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB500-126-0.025ml

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NBP1-31294

HDAC1 Antibody

100 ul

NBP1-31294

Knockout Validated: HDAC1 Antibody [NBP1-31294] - Wild-type (WT) and HDAC1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-31294] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC1 protein stained by HDAC1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.Blue: Hoechst 33342 staining

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. Green: HDAC1 antibody diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Chromatin Immunoprecipitation: HDAC1 Antibody [NBP1-31294] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of HDAC1 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

Western Blot: HDAC1 Antibody [NBP1-31294] - Sample: 1000 ug 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract, B. Control with 2. 5 ug of preimmune rabbit IgG, C. Immunoprecipitation of HDAC1 protein by 2. 5 ug of HDAC1 antibody 10% SDS-PAGE gel.

<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody [NBP1-31294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: HDAC1 Antibody [NBP1-31294] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: HDAC1 Antibody [NBP1-31294] - A. 30 ug Rat2 whole cell lysate/extract 10% SDS-PAGE HDAC1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody [NBP1-31294] - Analysis of A431, using HDAC1 antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Human testis, using HDAC1 antibody(10 ug/ml). Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Sample: Paraffin-embedded mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Rat brain. HDAC1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse liver. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP1-31294] - Mouse colon. HDAC1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Reactivity:

Hu, Mu, Rt, Ze, Bv

Species Glossary

Reactivity: Hu, Mu, Rt, Ze, Bv

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-16789

HDAC11 Antibody

(1 Review)

(1 Publication)

0.1 ml

NBP2-16789

Immunocytochemistry/Immunofluorescence: HDAC11 Antibody [NBP2-16789] - Sox2 gene expression is regulated by Gli1 and HDAC11. Double immunofuorescence assay with Gli1 and HDAC11 showed co-localization of both in A549 (upper panel) and H1650 (lower panel) cells. DAPI was used to stain the nucleus of the cells. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-020-61295-6) licensed under a CC-BY license.

Immunohistochemistry: HDAC11 Antibody [NBP2-16789] - HDAC11 expression in human lung tumor tissues and cells and its correlation with patient prognosis. Elevated HDAC11 staining is seen in NSCLC tissue and its metastatic sites as compared to the normal human lung tissue in TMA. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-020-61295-6) licensed under a CC-BY license.

Simple Western: HDAC11 Antibody [NBP2-16789] - 1:25 dilution against the indicated concentration of protein lysate of human SVG-A cells. Image submitted by a verified customer review.

<b>Genetic Strategies Validation. </b>Knockdown Validated: HDAC11 Antibody [NBP2-16789] - HDAC11 silencing decreases expression of downstream targets and abrogates self-renewal of CSCs. Depletion of HDAC11 using two IPTG inducible shRNA shows a significant decrease in Sox2, YAP1 and Gli1 protein expression as compared to IPTG treated control shRNA. The glycolysis pathway targets HK2 and PDK2 also show reduced protein expression in the absence of HDAC11. No change is observed in PDK1 expression; the full images of the western blots are provided as Supplementary Fig. 4. Image collected and cropped by CiteAb from the following publication (www.nature.com/articles/s41598-020-61295-6) licensed under a CC-BY license.

Western Blot: HDAC11 Antibody [NBP2-16789] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with HDAC11 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

HDAC11 silencing decreases expression of downstream targets and abrogates self-renewal of CSCs. (A) Depletion of HDAC11 using two IPTG inducible shRNA shows a significant decrease in Sox2, YAP1 and Gli1 protein expression as compared to IPTG treated control shRNA. The glycolysis pathway targets HK2 and PDK2 also show reduced protein expression in the absence of HDAC11. No change is observed in PDK1 expression; the full images of the western blots are provided as Supplementary Fig. 4. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32170113), licensed under a CC-BY licence.

Reactivity:

Hu, Mu, RM

Species Glossary

Reactivity: Hu, Mu, RM

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-03993

HDAC9 Antibody - BSA Free

(1 Review)

(1 Publication)

0.1 mg

NBP2-03993

Western Blot: HDAC9 Antibody [NBP2-03993] - Analysis of HDAC-9 in human HeLa cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) and mouse RAW cell lysate using this antibody. 0 ug/ml, 1.0 ug/ml and 0.5 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Reactivity:

Hu, Mu, Bv, Ca, Pm, Rt, Pm, Eq, Ma-Op, Pm-Or, RM

Species Glossary

Reactivity: Hu, Mu, Bv, Ca, Pm, Rt, Pm, Eq, Ma-Op, Pm-Or, RM

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6432A

Alt Catalog# IMG-6432A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-03993AF350
Alexa Fluor 405	NBP2-03993AF405
Alexa Fluor 488	NBP2-03993AF488
Alexa Fluor 532	NBP2-03993AF532
Alexa Fluor 594	NBP2-03993AF594
Alexa Fluor 647	NBP2-03993AF647
Alexa Fluor 700	NBP2-03993AF700
Alexa Fluor 750	NBP2-03993AF750
Allophycocyanin	NBP2-03993APC
Biotin	NBP2-03993B
DyLight 350	NBP2-03993UV
DyLight 405	NBP2-03993V
DyLight 488	NBP2-03993G
DyLight 550	NBP2-03993R
DyLight 594	NBP2-03993DL594
DyLight 650	NBP2-03993C
DyLight 680	NBP2-03993FR
DyLight 755	NBP2-03993IR
FITC	NBP2-03993F
HRP	NBP2-03993H
Janelia Fluor 549	NBP2-03993JF549
Janelia Fluor 646	NBP2-03993JF646
PE	NBP2-03993PE
PerCP	NBP2-03993PCP
mFluor Violet 450 SE	NBP2-03993MFV450
mFluor Violet 500 SE	NBP2-03993MFV500
mFluor Violet 610 SE	NBP2-03993MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-03993SS

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NB100-56343

HDAC6 Antibody - BSA Free

(2 Publications)

0.1 mg

NB100-56343

Western Blot: HDAC6 Antibody [NB100-56343] - Analysis of HDAC-6 in NIH-3T3 cell lysate with this antibody.

Immunocytochemistry/Immunofluorescence: HDAC6 Antibody [NB100-56343] - HeLa cells were fixed for 10 minutes using 4% PFA and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-HDAC6 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Simple Western: HDAC6 Antibody [NB100-56343] - Lane view shows a specific band for HDAC6 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-340

Alt Catalog# IMG-340

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-56343SS

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H00003065-M14

HDAC1 Antibody (5C11)

(1 Publication)

0.1 mg

H00003065-M14

Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M14), clone 5C11. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of monoclonal antibody to HDAC1 on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml

Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in PC-12 (Cat # L012V1).

Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in Hela S3 NE (Cat # L013V3).

Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in Raw 264.7 (Cat # L024V1).

Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in NIH/3T3 (Cat # L018V1).

ELISA: HDAC1 Antibody (5C11) [H00003065-M14] - Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.

Proximity Ligation Assay: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of protein-protein interactions between NFKB1 and HDAC1. HeLa cells were stained with anti-NFKB1 rabbit purified polyclonal 1:1200 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , , ,

Applications: , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP2-16791

HDAC3 Antibody

0.1 ml

NBP2-16791

Western Blot: HDAC3 Antibody [NBP2-16791] - HDAC3 antibody detects HDAC3 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted by 1:1000.

Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NBP2-16791] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 protein stained by HDAC3 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: HDAC3 Antibody [NBP2-16791] - Immunohistochemical analysis of paraffin-embedded OVCA, using antibody at 1:250 dilution.

Chromatin Immunoprecipitation: HDAC3 Antibody [NBP2-16791] - Cross-linked ChIP was performed with MCF-7 chromatin extract and 5 ug of either control rabbit IgG or anti-HDAC3 antibody. The precipitated DNA was detected by PCR with primer set targeting to TFF1 or HK1.

<b>Genetic Strategies Validation. </b>Western Blot: HDAC3 Antibody [NBP2-16791] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: HDAC3 Antibody [NBP2-16791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: HDAC3 Antibody [NBP2-16791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

Western Blot: HDAC3 Antibody [NBP2-16791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: HDAC3 Antibody [NBP2-16791] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunoprecipitation: HDAC3 Antibody [NBP2-16791] - 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of HDAC3 protein by 2 ug of HDAC3 antibody 7.5% SDS-PAGE The immunoprecipitated HDAC3 protein was detected by HDAC3 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.

Reactivity:

Hu, Mu, Rt, Ze, Po, Bv, Ch, RM

Species Glossary

Reactivity: Hu, Mu, Rt, Ze, Po, Bv, Ch, RM

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP2-52937

HDAC1 Antibody (CL0510)

0.1 ml

NBP2-52937

Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Analysis in human rectum and skeletal muscle tissues using NBP2-52937 antibody. Corresponding HDAC1 RNA-seq data are presented for the same tissues.

<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: HDAC1 Antibody (CL0510) [NBP2-52937] - analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HDAC1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of U-251 cells using the Anti-HDAC1 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Western Blot: HDAC1 Antibody (CL0510) [NBP2-52937] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human rectum shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-31196

HDAC3 Antibody

100 ul

NBP1-31196

Western Blot: HDAC3 Antibody [NBP1-31196] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NBP1-31196] - SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term diluted at 1:400. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 um.

Immunohistochemistry-Paraffin: HDAC3 Antibody [NBP1-31196] - SW480 xenograft, using HDAC3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Chromatin Immunoprecipitation: HDAC3 Antibody [NBP1-31196] - Sample: 293T whole cell lysate/extract A. 5 ug preimmune rabbit IgG, B. 5 ug of HDAC3 antibody The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

Western Blot: HDAC3 Antibody [NBP1-31196] - Analysis of on: A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 10% SDS PAGE diluted at 1:1000

Western Blot: HDAC3 Antibody [NBP1-31196] - Sample: 1000 ug 293T whole cell lysate/extract A. 50 ug 293T whole cell lysate/extract, B. Control with 2 ug of preimmune rabbit IgG, C. Immunoprecipitation of HDAC3 protein by 2 ug of HDAC3 antibody 10% SDS-PAGE gel.

<b>Genetic Strategies Validation. </b>Western Blot: HDAC3 Antibody [NBP1-31196] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: HDAC3 Antibody [NBP1-31196] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: HDAC3 Antibody [NBP1-31196] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with HDAC3 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [NBP1-31196] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HDAC3 protein stained by HDAC3 antibody [C3], C-term diluted at 1:500.Blue: Hoechst 33342 staining.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ch, RM

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ch, RM

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

compare

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