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Wnt-10a Antibody (342811)

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Western blot shows lysates of Raji human Burkitt's lymphoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Wnt-10a Monoclonal Antibody (Catalog # MAB34691) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Wnt-10a at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Wnt-10a Monoclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
342811
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB34691-SP
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Wnt-10a Antibody (342811) Summary

Immunogen
E. coli-derived recombinant mouse Wnt-10a
Leu55-Glu95 and Gln278-Arg360
Accession # P70701
Specificity
Detects mouse and human Wnt-10a in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Wnt-1, -2, -2b, -3a, -4, -5a, -5b, recombinant human (rh) Wnt-6 or rhWnt-7a is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
WNT10A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Wnt-10a Antibody (342811)

  • FLJ14301
  • protein Wnt-10a
  • SSPS
  • wingless-type MMTV integration site family, member 10A
  • Wnt10a
  • Wnt-10a

Background

Wnt-10a is a presumably secreted, glycosylated member of the Wnt family of developmental proteins. Mouse Wnt-10a is synthesized as a 417 amino acid (aa) precursor that contains a 382 aa mature region. The mature region contains 24 cysteines and two potential N-linked glycosylation sites. Mature mouse Wnt-10a shares 96% and 99% aa sequence identity with mature human and rat Wnt-10a, respectively. Wnt-10a shares 64% aa sequence identity with Wnt-10b/12. Wnt-10a is produced by tumors and thymic epithelium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Wnt-10a Antibody
(342811)
MAB34691
Wnt-10a Antibody (342811)
Species: Hu, Mu
Applications: WB

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Video Protocols

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Bioinformatics

Gene Symbol WNT10A
Uniprot