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Recombinant Human VPS4B His Protein

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SDS-Page: Recombinant Human VPS4B His Protein [NBP2-22937] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.25 mg/ml

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Recombinant Human VPS4B His Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-444 of Human VPS4B

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSSTSP NLQKAIDLAS KAAQEDKAGN YEEALQLYQH AVQYFLHVVK YEAQGDKAKQ SIRAKCTEYL DRAEKLKEYL KNKEKKAQKP VKEGQPSPAD EKGNDSDGEG ESDDPEKKKL QNQLQGAIVI ERPNVKWSDV AGLEGAKEAL KEAVILPIKF PHLFTGKRTP WRGILLFGPP GTGKSYLAKA VATEANNSTF FSISSSDLVS KWLGESEKLV KNLFQLAREN KPSIIFIDEI DSLCGSRSEN ESEAARRIKT EFLVQMQGVG VDNDGILVLG ATNIPWVLDS AIRRRFEKRI YIPLPEPHAR AAMFKLHLGT TQNSLTEADF RELGRKTDGY SGADISIIVR DALMQPVRKV QSATHFKKVR GPSRADPNHL VDDLLTPCSP GDPGAIEMTW MDVPGDKLLE PVVSMSDMLR SLSNTKPTVN EHDLLKLKKF TEDFGQEG

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
VPS4B
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Theoretical MW
51.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 30% glycerol, 1 mM DTT
Preservative
No Preservative
Concentration
0.25 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human VPS4B His Protein

  • Cell migration-inducing gene 1 protein
  • Protein SKD1
  • SKD1B
  • SKD1cell migration-inducing 1
  • Suppressor of K(+) transport growth defect 1
  • suppressor of K+ transport defect 1
  • vacuolar protein sorting 4 homolog B (S. cerevisiae)
  • vacuolar protein sorting 4B (yeast)
  • vacuolar protein sorting 4B
  • vacuolar protein sorting-associated protein 4B
  • VPS42
  • VPS4-2

Background

Vacuolar protein sorting-associated protein 4B, also known as VPS4B, belongs to the AAA (ATPases associated with diverse cellular activities) family. It is involved in late steps of the endosomal multivesicular bodies (MVB) pathway and recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Dominant negative mutant of VPS4B inhibit vacuolar protein sorting and also arrest HIV-1 and MLV budding. Recombinant human VPS4B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol VPS4B