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Rat Vitamin D BP ELISA Kit (Colorimetric)

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ELISA: Rat Vitamin D BP ELISA Kit (Colorimetric) [NBP2-67930] - Samples were spiked with high concentrations of Rat Vitamin D BP and diluted with Reference Standard & Sample Diluent to produce samples with values within ...read more
ELISA: Rat Vitamin D BP ELISA Kit (Colorimetric) [NBP2-67930] - Standard Curve Reference

Product Details

Summary
Reactivity RtSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma and other biological fluids
Standard Curve Range
15.63 - 1000 ng/mL
Sensitivity
9.38 ng/mL

Order Details

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Rat Vitamin D BP ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Rat DBP in samples. No significant cross-reactivity or interference between Rat DBP and analogues was observed.
Standard Curve Range
15.63 - 1000 ng/mL
Sensitivity
9.38 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.85%
Intra-Assay
CV% < 4.76%
Spike Recovery
91-110%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
GC

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Rat Vitamin D BP ELISA Kit (Colorimetric)

  • DBP
  • DBP/GC
  • DBP-MAF
  • DBPVDBG
  • EC 6.3.1.5
  • Gc
  • gc-globulin
  • Gc-MAF
  • GRD3
  • group-specific component (vitamin D binding protein)
  • Group-specific component
  • hDBP
  • VDB
  • VDBG
  • VDBP
  • Vitamin D BP
  • vitamin D-binding alpha-globulin
  • vitamin D-binding protein

Background

Vitamin D Binding Protein is a secreted multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and urine, and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane bound immunoglobulin on the surface of B lymphocytes and with IgG fc receptor on the membranes of T lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GC