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Vitamin D BP Antibody (VDBP/4482) - Azide and BSA Free

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Protein Array: Vitamin D BP Antibody (VDBP/4482) - Azide and BSA Free [NBP3-14006] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vitamin D BP Antibody (VDBP/4482).
Protein Array: Vitamin D BP Antibody (VDBP/4482) - Azide and BSA Free [NBP3-14006] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Vitamin D BP Antibody (VDBP/4482).
IHC analysis of formalin-fixed, paraffin-embedded human tonsil. Staining using Vitamin D BP antibody (VDBP/4482) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, MA
Clone
VDBP/4482
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-14006AF350
Alexa Fluor 405
NBP3-14006AF405
Alexa Fluor 488
NBP3-14006AF488
Alexa Fluor 532
NBP3-14006AF532
Alexa Fluor 594
NBP3-14006AF594
Alexa Fluor 647
NBP3-14006AF647
Alexa Fluor 700
NBP3-14006AF700
Alexa Fluor 750
NBP3-14006AF750
Allophycocyanin
NBP3-14006APC
Biotin
NBP3-14006B
CoraFluor 1
NBP3-14006CL1
DyLight 350
NBP3-14006UV
DyLight 405
NBP3-14006V
DyLight 488
NBP3-14006G
DyLight 550
NBP3-14006R
DyLight 594
NBP3-14006DL594
DyLight 650
NBP3-14006C
DyLight 680
NBP3-14006FR
DyLight 755
NBP3-14006IR
FITC
NBP3-14006F
HRP
NBP3-14006H
Janelia Fluor 525
NBP3-14006JF525
Janelia Fluor 549
NBP3-14006JF549
Janelia Fluor 585
NBP3-14006JF585
Janelia Fluor 635
NBP3-14006JF635
Janelia Fluor 646
NBP3-14006JF646
Janelia Fluor 669
NBP3-14006JF669
PE
NBP3-14006PE
PerCP
NBP3-14006PCP
Unconjugated
NBP3-13924-100ug
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-13924-100ug
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Vitamin D BP Antibody (VDBP/4482) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13924).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Immunogen
Recombinant fragment (around aa 35-175) of human Vitamin D BP protein (exact sequence is proprietary) (Uniprot: P02774)
Localization
Secreted
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GC
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for Vitamin D BP Antibody (VDBP/4482) - Azide and BSA Free

  • DBP
  • DBP/GC
  • DBP-MAF
  • DBPVDBG
  • EC 6.3.1.5
  • Gc
  • gc-globulin
  • Gc-MAF
  • GRD3
  • group-specific component (vitamin D binding protein)
  • Group-specific component
  • hDBP
  • VDB
  • VDBG
  • VDBP
  • Vitamin D BP
  • vitamin D-binding alpha-globulin
  • vitamin D-binding protein

Background

Vitamin D Binding Protein is a secreted multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and urine, and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane bound immunoglobulin on the surface of B lymphocytes and with IgG fc receptor on the membranes of T lymphocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Vitamin D BP
Antibody (VDBP/4482) ...
NBP3-14006
Vitamin D BP Antibody (VDBP/4482) - Azide and BSA Free
Species: Hu
Applications: IHC, MA

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Bioinformatics

Gene Symbol GC