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VEGFR3/Flt-4 Antibody (9D9) [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
9D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-18651AF350
Alexa Fluor 405
NBP1-18651AF405
Alexa Fluor 488
NBP1-18651AF488
Alexa Fluor 532
NBP1-18651AF532
Alexa Fluor 594
NBP1-18651AF594
Alexa Fluor 647
NBP1-18651AF647
Alexa Fluor 700
NBP1-18651AF700
Alexa Fluor 750
NBP1-18651AF750
Allophycocyanin
NBP1-18651APC
CoraFluor 1
NBP1-18651CL1
DyLight 350
NBP1-18651UV
DyLight 405
NBP1-18651V
DyLight 488
NBP1-18651G
DyLight 550
NBP1-18651R
DyLight 594
NBP1-18651DL594
DyLight 650
NBP1-18651C
DyLight 680
NBP1-18651FR
DyLight 755
NBP1-18651IR
FITC
NBP1-18651F
HRP
NBP1-18651H
Janelia Fluor 525
NBP1-18651JF525
Janelia Fluor 549
NBP1-18651JF549
Janelia Fluor 646
NBP1-18651JF646
Janelia Fluor 669
NBP1-18651JF669
PE
NBP1-18651PE
PerCP
NBP1-18651PCP
Unconjugated
NBP1-18651
mFluor Violet 450 SE
NBP1-18651MFV450
mFluor Violet 500 SE
NBP1-18651MFV500
mFluor Violet 610 SE
NBP1-18651MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-18651
Azide and BSA Free
NBP1-18651
Azide and BSA Free
NBP1-18651-0.02mg
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VEGFR3/Flt-4 Antibody (9D9) [Biotin] Summary

Description
Clone #1 changed to 9D9 as a unique identifier.
Immunogen
Recombinant human soluble VEGFR-3/FLT-4
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
FLT4
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • ELISA 1-10 ug/ml
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 1-5 ug/ml
  • Western Blot 2-5 ug/ml
Application Notes
ELISA: To detect human VEGFR-3/FLT-4 by direct ELISA a concentration of 0.5 - 1.5 ug/ml can be used. This purified IgG, in combination with compatible secondary reagents, allows the detection of 0.25- 0.5 ng/well rhVEGFR-3/FLT-4. For Western blot analysis, the antibody can be used at 0.5 - 1 ug/ml with the appropriate secondary reagents to detect human VEGFR-3/FLT-4. Depending on the visualisation method the detection limit for rh VEGFR-3/FLT-4 is approximately 5 ng/lane under reducing conditions.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50X BSA
Preservative
0.02% Sodium Azide
Concentration
LYOPH
Purity
Protein A purified
Reconstitution Instructions
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.

Alternate Names for VEGFR3/Flt-4 Antibody (9D9) [Biotin]

  • EC 2.7.10
  • EC 2.7.10.1
  • FLT4
  • Flt-4
  • fms-related tyrosine kinase 4
  • LMPH1A
  • PCLFLT41
  • soluble VEGFR3 variant 1
  • soluble VEGFR3 variant 2
  • soluble VEGFR3 variant 3
  • Tyrosine-protein kinase receptor FLT4
  • vascular endothelial growth factor receptor 3
  • VEGF R3
  • VEGFR3
  • VEGFR-3
  • VEGFR3Fms-like tyrosine kinase 4

Background

Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine esidues and sharing common VEGF receptors. VEGFs stimulate the proliferation of endothelial cells, induce angiogenesis, and increase vascular permeability in both large and small vessels. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors. VEGF Receptor 3 is one of the five receptor tyrosine kinases (RTKs) (VEGF Receptor 1/Flt1, VEGF Receptor 2/KDR/Flk1, VEGF Receptor 3/Flt4, tie1 and tek/tie2) whose expression is almost exclusively restricted to endothelial cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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VEGFR3/Flt-4
Antibody (9D9) [Bioti
NBP1-18652
VEGFR3/Flt-4 Antibody (9D9) [Biotin]
Species: Hu
Applications: WB, ELISA, ICC/IF, IP

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FLT4