Novus Biologicals products are now on bio-techne.com

UBE2T Antibody

Images

 
Image 1 of 1
(
Enlarge)
Western Blot: UBE2T Antibody [NB100-1150] - Analysis of UBE2T in K562 lysate (35ug protein in RIPA buffer) using NB100-1150 at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

  • Size / Catalog#QtyPrice
    NB100-1150
    0.1 mg
    $399.00
    2-4 Business Days
Shipping $45.00 per order
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Datasheet
Reviews & Publications
Protocols & FAQs
Support & Research

UBE2T Antibody Summary

Immunogen
Peptide with sequence C-QLVGIEKKFHPDV corresponding to C-Terminus according to NP_054895.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
UBE2T
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 22 kDa band observed in lysates of K562, HEK293 and A549 (calculated MW of 22.5 kDa band according to NP_054895.1).
Control
UBE2T Overexpression Lysate
Publications
Read Publication using NB100-1150.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for UBE2T Antibody

  • Cell proliferation-inducing gene 50 protein
  • EC 6.3.2.19
  • HSPC150 protein similar to ubiquitin-conjugating enzyme
  • HSPC150
  • PIG50
  • UBE2T
  • Ubiquitin carrier protein T
  • ubiquitin conjugating enzyme
  • ubiquitin-conjugating enzyme E2 T
  • ubiquitin-conjugating enzyme E2T (putative)
  • Ubiquitin-protein ligase T

Background

The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 [PubMed 16916645]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-89522
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-89150
Species: Hu, Rt
Applications: IHC, IHC-P, WB
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information


Product PDFs

  SDS

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our UBE2T Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol UBE2T
Entrez
Uniprot