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UBE2T Antibody (OTI2F5) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clone
OTI2F5
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

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UBE2T Antibody (OTI2F5) [Janelia Fluor® 669] Summary

Immunogen
Full length human recombinant protein of human UBE2T (NP_054895) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
UBE2T
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for UBE2T Antibody (OTI2F5) [Janelia Fluor® 669]

  • Cell proliferation-inducing gene 50 protein
  • EC 6.3.2.19
  • HSPC150 protein similar to ubiquitin-conjugating enzyme
  • HSPC150
  • PIG50
  • UBE2T
  • Ubiquitin carrier protein T
  • ubiquitin conjugating enzyme
  • ubiquitin-conjugating enzyme E2 T
  • ubiquitin-conjugating enzyme E2T (putative)
  • Ubiquitin-protein ligase T

Background

The covalent conjugation of ubiquitin to proteins regulates diverse cellular pathways and proteins. Ubiquitin is transferred to a target protein through a concerted action of a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), such as UBE2T, and a ubiquitin ligase (E3) (Machida et al., 2006 (PubMed 16916645)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol UBE2T