![Western Blot: UBE2T Antibody (1E12-4A3) [H00029089-M01] - UBE2T monoclonal antibody (M01), clone 1E12-4A3 Analysis of UBE2T expression in Hela.](http://images.novusbio.com/fullsize/UBE2T-Antibody-1E12-4A3-Western-Blot-H00029089-M01-img0003.jpg "Western Blot: UBE2T Antibody (1E12-4A3) [H00029089-M01] - UBE2T monoclonal antibody (M01), clone 1E12-4A3 Analysis of UBE2T expression in Hela.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, IP |
| Clone | 1E12-4A3 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | UBE2T (AAH04152, 1 a.a. ~ 197 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQRASRLKRELHMLATEPPPGITCWQDKDQMDDLRAQILGGANTPYEKGVFKLEVIIPERYPFEPPQIRFLTPIYHPNIDSAGRICLDVLKLPPKGAWRPSLNIATVLTSIQLLMSEPNPDDPLMADISSEFKYNKPAFLKNARQWTEKHARQKQKADEEEMLDNLPEAGDSRVHNSTQKRKASQLVGIEKKFHPDV |
| Specificity | UBE2T - ubiquitin-conjugating enzyme E2T (putative) |
| Isotype | IgG2b Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | UBE2T |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA. |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
| Publication using H00029089-M01 | Applications | Species |
|---|---|---|
| Ramaekers CH, van den Beucken T, Meng A et al. Hypoxia disrupts the Fanconi anemia pathway and sensitizes cells to chemotherapy through regulation of UBE2T. Radiother Oncol. 2011-06-29 [PMID: 21722982] |
Secondary Antibodies |
Isotype Controls |
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![Immunoprecipitation: UBE2T Antibody (1E12-4A3) [H00029089-M01] - Analysis of UBE2T transfected lysate using anti-UBE2T monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with UBE2T MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/UBE2T-Antibody-1E12-4A3-Immunoprecipitation-H00029089-M01-img0002.jpg "Immunoprecipitation: UBE2T Antibody (1E12-4A3) [H00029089-M01] - Analysis of UBE2T transfected lysate using anti-UBE2T monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with UBE2T MaxPab rabbit polyclonal antibody.")
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