![Western Blot: Tuftelin 1 Antibody [NBP1-87446] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/Tuftelin-1-Antibody-Western-Blot-NBP1-87446-img0007.jpg "Western Blot: Tuftelin 1 Antibody [NBP1-87446] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).")
| Reactivity | Hu, RtSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: DCVAFEVTLSRYQREAEQSNVALQREEDRVEQKEAEVGELQRRLLGMETEHQALLAKVREGEVALEELRSNNADCQAEREKAATLEKEVAGLREKIHHL |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TUFT1 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
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| Theoretical MW | 44 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control Peptide |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
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| Publication using NBP1-87446 | Applications | Species |
|---|---|---|
| Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunohistochemistry-Paraffin: Tuftelin 1 Antibody [NBP1-87446] - Staining of human tonsil shows weak cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Tuftelin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87446-img0012.jpg "Immunohistochemistry-Paraffin: Tuftelin 1 Antibody [NBP1-87446] - Staining of human tonsil shows weak cytoplasmic positivity in squamous epithelial cells.")
![Western Blot: Tuftelin 1 Antibody [NBP1-87446] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-614](http://images.novusbio.com/fullsize/Tuftelin-1-Antibody-Western-Blot-NBP1-87446-img0006.jpg "Western Blot: Tuftelin 1 Antibody [NBP1-87446] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-614")
![Immunohistochemistry-Paraffin: Tuftelin 1 Antibody [NBP1-87446] - Staining of human skin shows moderate positivity in a subset of Keratinocytes.](http://images.novusbio.com/fullsize/Tuftelin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87446-img0008.jpg "Immunohistochemistry-Paraffin: Tuftelin 1 Antibody [NBP1-87446] - Staining of human skin shows moderate positivity in a subset of Keratinocytes.")
![Immunohistochemistry-Paraffin: Tuftelin 1 Antibody [NBP1-87446] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Tuftelin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87446-img0009.jpg "Immunohistochemistry-Paraffin: Tuftelin 1 Antibody [NBP1-87446] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.")
![Immunohistochemistry-Paraffin: Tuftelin 1 Antibody [NBP1-87446] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Tuftelin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87446-img0010.jpg "Immunohistochemistry-Paraffin: Tuftelin 1 Antibody [NBP1-87446] - Staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.")
![Immunohistochemistry-Paraffin: Tuftelin 1 Antibody [NBP1-87446] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Tuftelin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87446-img0011.jpg "Immunohistochemistry-Paraffin: Tuftelin 1 Antibody [NBP1-87446] - Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")