TSLP ELISA Development Kit Summary
Standard Curve Range |
0.31 - 20ng/ml |
Sensitivity |
1 ng/ml |
Assay Type |
Sandwich ELISA |
Sample Volume |
100 uL |
Kit Type |
ELISA Development Kit |
Gene |
TSLP |
Applications/Dilutions
Dilutions |
|
Application Notes |
This is an enzyme-linked immunosorbent assay for quantitative detection. |
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Capture Antibody: 0.5 mg/mL of mouse anti-TSLP monoclonal antibody (clone 209A2.03). Dilute in carbonate buffer pH9.6 to a working concentration of 2.5 ug /mL
- Detection Antibody: 0.5mg/mL of Biotin-conjugated anti-TSLP mouse monoclonal antibody (clone 210H1.04). Dilute to a working concentration of 3ug/mL in PBS-BSA1%-Tween 0.05%.
- Standard: Each vial contains 1ug/mL of recombinant TSLP produced, purified and concentrated from eukaryotic cells. A 7-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 20 ng /mL is recommended
|
Notes
This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.
Alternate Names for TSLP ELISA Development Kit
Background
TSLP (thymic stromal lymphopoietin) is a hemopoietic protein of 159 aa (18 kDa), produced mainly by non-hematopoietic cells such as fibroblasts, epithelial cells and different types of stromal or stromallike cells. It is known to play an important role in the maturation of T cell populations through activation of antigen presenting cells. It is also detected in the thymus and the tissue cells of the bone marrow. TSLP affects the transition from pre-B cells in B. Alternative splicing of this gene results in two transcripts variants. TSLP is proposed to signal through a heterodimeric receptor complex composed of TSLP-receptor and IL7R chain. Expressed in many tissues, it prevents apoptosis and stimulates the growth of myeloid cells. (Reche PA et al, J Immunol., 2001, 167(1):336-43; Dorshkind. K, Nature. Immunol., 2000 (1):369-370). Monoclonal antibodies raised against human TSLP were obtained after mice immunization with TSLP-His-tagged transfected 293T cells, and were used to set up a quantitative immunoassay to determine TSLP concentration in biological samples
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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