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TSG101 Antibody (1065916) [Unconjugated]

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Western blot shows lysates of A172 human glioblastoma cell line, MDA‑MB‑231 human breast cancer cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human TSG101 Monoclonal Antibody (Catalog # MAB114381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TSG101 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Western blot shows lysates of A172 human glioblastoma cell line, MDA‑MB‑231 human breast cancer cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/ml of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
1065916
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB114381-SP
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TSG101 Antibody (1065916) [Unconjugated] Summary

Immunogen
TSG101 containing synthetic peptide
Accession # Q99816
Specificity
Detects TSG101 in direct ELISA.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSG101 Antibody (1065916) [Unconjugated]

  • ESCRT-I complex subunit TSG101
  • TSG10
  • TSG101
  • tumor susceptibility gene 10
  • tumor susceptibility gene 101 protein
  • tumor susceptibility gene 101
  • tumor susceptibility protein
  • VPS23

Background

TSG101 is an essential member of the ESCRT-1 complex which regulates the sorting of ubiquitinated proteins to endosomes, facilitating vesicular trafficking and is implicated in normal development.  It is also involved in regulating transcription, protein sorting, biogenesis of multi-vesicular bodies, and viral budding.  Dysregulation of ESCRT proteins occurs in the development of various human diseases, including many types of cancers and neurodegenerative diseases.  TSG101 is an established cancer-associated gene and truncated aberrantly spliced mRNAs have been reported in various types of cancer. TSG101 is also commonly used as a marker protein for exosomes.
  1. Tufan AB, Lazarow K, Kolesnichenko M, Sporbert A, von Kries JP, Scheidereit C. TSG101 associates with PARP1 and is essential for PARylation and DNA damage-induced NF-kappa B activation. EMBO J. 2022 Nov 2;41(21):e110372.
    doi: 10.15252/embj.2021110372. Epub 2022 Sep 20. PMID: 36124865; PMCID: PMC9627669. 
  2. Chua HH, Kameyama T, Mayeda A, Yeh TH. Cancer-Specifically Re-Spliced TSG101 mRNA Promotes Invasion and Metastasis of Nasopharyngeal Carcinoma. Int J Mol Sci. 2019 Feb 12;20(3):773.
    doi: 10.3390/ijms20030773. PMID: 30759747; PMCID: PMC6387056. 
  3. Willms E, Johansson HJ, Mäger I, Lee Y, Blomberg KE, Sadik M, Alaarg A, Smith CI, Lehtiö J, El Andaloussi S, Wood MJ, Vader P. Cells release subpopulations of exosomes with distinct molecular and biological properties. Sci Rep. 2016 Mar 2;6:22519.
    doi: 10.1038/srep22519. PMID: 26931825; PMCID: PMC4773763.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TSG101 Antibody
(1065916) [Unconjugat ...
MAB114381
TSG101 Antibody (1065916) [Unconjugated]
Species: Hu
Applications: WB

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