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TSEN2 Antibody

Images

 
Western Blot: TSEN2 Antibody [NBP1-81140] - Analysis in control (vector only transfected HEK293T lysate) and TSEN2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: TSEN2 Antibody [NBP1-81140] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TSEN2 Antibody [NBP1-81140] - Staining of human cerebellum shows nuclear positivity in Purkinje cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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TSEN2 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: GCHPTTESFEKSVREDASPLPHVCCCKQDALILQRGLHHEDGSQHIGLLHPGDRGPDHEYVLVEEAECAMSEREAAPNEELVQRNRLICRR
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TSEN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TSEN2 Protein (NBP1-81140PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TSEN2 Antibody

  • EC 3.1.27.9
  • hsSen2
  • MGC2776
  • PCH2B
  • SEN2L
  • SEN2MGC4440
  • tRNA splicing endonuclease 2 homolog (S. cerevisiae)
  • tRNA splicing endonuclease 2 homolog (SEN2, S. cerevisiae)
  • tRNA-intron endonuclease Sen2
  • tRNA-splicing endonuclease subunit Sen2

Background

tRNA splicing is a fundamental process required for cell growth and division. SEN2 is a subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns, the first step in tRNA splicing (Paushkin et al., 2004 [PubMed 15109492]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TSEN2
Entrez