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Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody

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Western Blot: Tryptophan Hydroxylase 1 [p Ser58] Antibody [NB300-260] - Recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of cAMP-dependent protein kinase (PKA) showing specific ...read more

Product Details

Summary
Reactivity Mu, Rb, Hu, Mu, Rt, Bv, Ca, Ch, Pm, Xp, ZeSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody Summary

Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH. Accession # P09810
Modification
p Ser58
Localization
Cytoplasmic
Specificity
Specific for endogenous levels of the ~55 kDa Tryptophan Hydroxylase 1/TPH-1 protein phosphorylated at Ser58.
Predicted Species
Human (100%), Mouse (100%), Rat (100%), Chicken (100%), Canine (100%), Xenopus (100%), Primate (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TPH1
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state expression levels.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Tryptophan Hydroxylase 1/TPH-1 Overexpression Lysate

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Antigen Affinity-purified

Alternate Names for Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody

  • EC 1.14.16
  • EC 1.14.16.4
  • indoleacetic acid-5-hydroxylase
  • L-tryptophan hydroxylase
  • MGC119994
  • TPH
  • TPH-1
  • TPRH
  • TRPH
  • tryptophan 5-hydroxylase 1
  • Tryptophan 5-monooxygenase 1
  • tryptophan hydroxylase (tryptophan 5-monooxygenase)
  • Tryptophan Hydroxylase 1

Background

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol TPH1
Entrez
Uniprot