Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody Summary
Immunogen |
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH. Accession # P09810 |
Modification |
p Ser58 |
Localization |
Cytoplasmic |
Specificity |
Specific for endogenous levels of the ~55 kDa Tryptophan Hydroxylase 1/TPH-1 protein phosphorylated at Ser58. |
Predicted Species |
Human (100%), Mouse (100%), Rat (100%), Chicken (100%), Canine (100%), Xenopus (100%), Primate (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
TPH1 |
Purity |
Antigen Affinity-purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
|
Application Notes |
Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state expression levels. |
Theoretical MW |
55 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Control |
|
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol |
Preservative |
No Preservative |
Purity |
Antigen Affinity-purified |
Alternate Names for Tryptophan Hydroxylase 1/TPH-1 [p Ser58] Antibody
Background
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CAM K II). Both PKA and CAM K II phosphorylate Ser58 which lies within the regulatory domain of TPH.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu, Mu, Pm, Rb, Rt
Applications: Flow, ICC/IF, IHC-FrFl, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu, Mu
Applications: WB
Species: Hu, Mu
Applications: IHC, IHC-P, WB
Species: Ch, Dr, Hu, I, Ma, Mu, Rt
Applications: Dual ISH-IHC, ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: IHC, IHC-P, WB
Species: Hu, Mu
Applications: Flow, PEP-ELISA, WB
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Species: Hu, Mu
Applications: ICC/IF, IHC-Fr, Simple Western, WB
Species: Hu
Applications: BA
Species: Hu, Mu, Rt
Applications: ICC/IF, WB
Species: Hu, Mu
Applications: IHC, IHC-P, WB
Species: Hu, Mu, Rt
Applications: ICC, IHC, IP, WB
Species: Hu, Pm, Pm
Applications: IHC, IHC-P
Species: Hu
Applications: ELISA
Species: Hu
Applications: CyTOF-ready, Flow, IHC
Species: Hu, Mu, Rt
Applications: WB
Species: Hu, Mu, Rt
Applications: WB
Species: Mu, Rt
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), IHC, Neut, Simple Western, WB
Species: Mu, Rb, Hu, Mu, Rt, Bv, Ca, Ch, Pm, Xp, Ze
Applications: WB
Publications for Tryptophan Hydroxylase 1/TPH-1 Antibody (NB300-260) (0)
There are no publications for Tryptophan Hydroxylase 1/TPH-1 Antibody (NB300-260).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for Tryptophan Hydroxylase 1/TPH-1 Antibody (NB300-260) (0)
There are no reviews for Tryptophan Hydroxylase 1/TPH-1 Antibody (NB300-260).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Tryptophan Hydroxylase 1/TPH-1 Antibody (NB300-260) (0)
Control Lysate(s)
Secondary Antibodies
| |
Isotype Controls
|
Additional Tryptophan Hydroxylase 1/TPH-1 Products
Research Areas for Tryptophan Hydroxylase 1/TPH-1 Antibody (NB300-260)
Find related products by research area.
|
Blogs on Tryptophan Hydroxylase 1/TPH-1