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Tryptase gamma-1/TPSG1 Antibody (279005)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clone
279005
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB1667-SP
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Tryptase gamma-1/TPSG1 Antibody (279005) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Tryptase gamma ‑1/TPSG1
Arg20-Arg281
Accession # Q9NRR2
Specificity
Detects human Tryptase gamma ‑1/TPSG1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) MCPT-1, recombinant human (rh) Tryptase alpha , rmTryptase beta -1, rhTryptase beta -2, or rmTryptase-5 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
TPSG1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
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Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tryptase gamma-1/TPSG1 Antibody (279005)

  • EC 3.4.21
  • EC 3.4.21.-
  • gamma I
  • gamma II
  • lung tryptase
  • mast cell protease II
  • mast cell tryptase
  • PRSS31skin tryptase
  • Serine protease 31
  • TMTpituitary tryptase
  • TPSG1
  • Transmembrane tryptase
  • trpA
  • tryptase gamma 1
  • tryptase gamma I
  • tryptase gamma II
  • tryptase gamma
  • Tryptase gamma1
  • Tryptase gamma-1

Background

As mediators of inflammatory and allergic response, mast cells are found throughout the body concentrated near blood vessels in connective tissue and the mucous membranes of the respiratory and gastrointestinal tract (3). Upon activation, human mast cells release granules that are enriched with neutral serine proteases including tryptases, chymase and cathepsin G (4). Tryptase gamma -1, also called transmembrane tryptase, is encoded by TPSG1, one of many serine protease genes clustered in human chromosome 16p13.3 (5). Human Tryptase gamma ‑1 is synthesized as a 321 amino acid preproenzyme with a C-terminal transmembrane anchor (1, 2).

  1. Wong, G.W. et al. (1999) J. Biol. Chem. 274:30784.
  2. Caughey, G.H. et al. (2000) J. Immunol. 164:6566.
  3. Harris, J.L. et al. (2001) J. Biol. Chem. 276:34941.
  4. Miller, H.R.P. and A.D. Pemberton (2002) Immunology 105:375.
  5. Wong, G.W. et al. (2002) J. Biol. Chem. 277:41906.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TPSG1
Uniprot