Troponin I type 2 (fast skeletal) Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: Troponin I type 2 (fast skeletal) Antibody [NBP2-38775] - Staining in human skeletal muscle and heart muscle tissues using anti-TNNI2 antibody. Corresponding ...read more
Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-38775] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. ...read more
Immunocytochemistry/ Immunofluorescence: Troponin I type 2 (fast skeletal) Antibody [NBP2-38775] - Staining of human cell line hTCEpi shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Troponin I type 2 (fast skeletal) Antibody [NBP2-38775] - Staining of human heart muscle shows low expression as expected.
Immunohistochemistry-Paraffin: Troponin I type 2 (fast skeletal) Antibody [NBP2-38775] - Staining of human skeletal muscle shows high expression.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Troponin I type 2 (fast skeletal) Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ELEKEESRREAEKQNYLAEHCPPLHIPGSMSEVQELCKQLHAKIDAAEEEKYDMEVRVQKTSK
Predicted Species
Mouse (90%), Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TNNI2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:2500 - 1:5000
  • Immunohistochemistry-Paraffin 1:2500 - 1:5000
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Troponin I type 2 (fast skeletal) Protein (NBP2-38775PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Troponin I type 2 (fast skeletal) Antibody

  • skeletal, fast
  • troponin I type 2 (skeletal, fast)
  • troponin I, fast skeletal muscle
  • Troponin I, fast-twitch isoform
  • troponin I, fast-twitch skeletal muscle isoform

Background

The troponin I type 2 gene encodes a fast-twitch skeletal muscle protein, a member of the troponin I gene family, and a component of the troponin complex including troponin T, troponin C and troponin I subunits. The troponin complex, along with tropomyosin, is responsible

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Research Areas for Troponin I type 2 (fast skeletal) Antibody (NBP2-38775)

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Blogs on Troponin I type 2 (fast skeletal).

Troponin I Type 2 - I stay with fast-twitch skeletal muscles only
The protein Troponin I is a component of the heteromeric protein complex responsible for regulating both skeletal and cardiac muscle contraction. Troponin complex is made up of three parts: troponin I (TnI), troponin T (TnT), and troponin C (TnC). Eac...  Read full blog post.

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Bioinformatics

Gene Symbol TNNI2
Uniprot