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TRIT1 Antibody

Images

 
Western Blot: TRIT1 Antibody [NBP1-82210] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: TRIT1 Antibody [NBP1-82210] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TRIT1 Antibody [NBP1-82210] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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TRIT1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LEPALEIVQSFIQGHKPTATPIKMPYNEAENKRSYHLCDLCDRIIIGDREWAAHIKSKSHLNQLKKRRRLDSDAVNTIESQSVSPDH
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
TRIT1 Protein (NBP1-82210PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TRIT1 Antibody

  • EC 2.5.1.75
  • EC 2.5.1.8
  • FLJ20061
  • hGRO1
  • IPP transferase
  • IPTase
  • IPTMGC149243
  • MGC149242
  • mitochondrial
  • MOD5
  • tRNA isopentenylpyrophosphate transferase
  • tRNA isopentenyltransferase 1
  • tRNA isopentenyltransferase

Background

TRIT1 catalyzes the transfer of a farnesyl moiety from farnesyl pyrophosphate to a cysteine at the fourth positionfrom the C-terminus of several proteins. The beta subunit is responsible for peptide-binding

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol TRIT1