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Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free

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Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SM22 alpha (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SM22 alpha.
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SM22 alpha (Right lane) cDNA for 48 ...read more
Immunocytochemistry/ Immunofluorescence: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY
Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584] - Staining of paraffin-embedded Human prostate tissue using anti-SM22 alpha mouse monoclonal antibody.
Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SM22 alpha monoclonal antibody.
Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SM22 alpha mouse monoclonal ...read more
Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SM22 alpha mouse monoclonal antibody.
Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SM22 alpha mouse monoclonal antibody.
Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SM22 alpha mouse monoclonal antibody.
Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584] - Staining of paraffin-embedded Human breast tissue using anti-SM22 alpha mouse monoclonal antibody.
Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584] - Staining of paraffin-embedded Human endometrium tissue using anti-SM22 alpha mouse monoclonal antibody.
Immunohistochemistry: Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free [NBP2-74584] - Staining of paraffin-embedded human breast cancer tissue using anti-TAGLN mouse monoclonal antibody. (Heat-induced ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI8C8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74584AF350
Alexa Fluor 405
NBP2-74584AF405
Alexa Fluor 488
NBP2-74584AF488
Alexa Fluor 532
NBP2-74584AF532
Alexa Fluor 594
NBP2-74584AF594
Alexa Fluor 647
NBP2-74584AF647
Alexa Fluor 700
NBP2-74584AF700
Alexa Fluor 750
NBP2-74584AF750
Biotin
NBP2-74584B
CoraFluor 1
NBP2-74584CL1
DyLight 350
NBP2-74584UV
DyLight 405
NBP2-74584V
DyLight 488
NBP2-74584G
DyLight 550
NBP2-74584R
DyLight 594
NBP2-74584DL594
DyLight 650
NBP2-74584C
DyLight 680
NBP2-74584FR
DyLight 755
NBP2-74584IR
FITC
NBP2-74584F
HRP
NBP2-74584H
Janelia Fluor 525
NBP2-74584JF525
Janelia Fluor 549
NBP2-74584JF549
Janelia Fluor 585
NBP2-74584JF585
Janelia Fluor 635
NBP2-74584JF635
Janelia Fluor 646
NBP2-74584JF646
Janelia Fluor 669
NBP2-74584JF669
Unconjugated
NBP2-00894
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-00894
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Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free Summary

Immunogen
Full length human reocombinant protein of human TAGLN(NP_003177) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TAGLN
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-2000
Theoretical MW
22.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Transgelin/TAGLN/SM22 alpha Antibody (OTI8C8) - Azide and BSA Free

  • DKFZp686P11128
  • Protein WS3-10
  • SM22 alpha
  • SM2222 kDa actin-binding protein
  • SMCCDKFZp686B01212
  • Smooth muscle protein 22-alpha
  • TAGLN
  • TAGLN1
  • transgelin variant 2
  • Transgelin
  • WS3-10
  • WS3-10SM22-alpha

Background

In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22 kDa protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus.The protein is a transformation and shape-change sensitive actin cross-linking/geling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TAGLN