Reactivity | MuSpecies Glossary |
Applications | WB, ELISA, IHC, PAGE |
Description | Native Mouse Transferrin Source: Normal serum (Accession #:Q921I1) Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Mouse Transferrin is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
Preparation Method |
This product was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation followed by extensive dialysis against the buffer stated above. Mouse Transferrin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Transferrin and anti-Mouse Serum. |
Protein/Peptide Type | Native Protein |
Gene | TF |
Purity | Multi-step |
Dilutions |
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Application Notes | This product is supplied in 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH: 7.6. Mouse Transferrin has been tested by SDS-PAGE and is a suitable protein for use as a control reagent in SDS, Western Blotting, and ELISA experiments. For blotting, the expected molecular weight of Mouse Transferrin is approximately 80kDa. Mouse Transferrin, when unopened, is stable at 4C, but for extended use should be aliquoted and stored at -20C in its undiluted form. If dilutions of Mouse Transferrin are required, they should be prepared just prior to use and not retained. |
Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH: 7.6 |
Preservative | No Preservative |
Purity | Multi-step |
Research Areas for Transferrin Native Protein (NBP1-97006)Find related products by research area.
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Transferrin: "Ironing" out the Details of Cellular Anemia Transferrin is a protein found in the blood plasma, a glycoprotein that is specific for controlling free iron in the bodies' biological fluids. Transferrin has two binding sites that are specific for very tight and reversible iron binding. The binding... Read full blog post. |
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