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Transcription factor E3 Antibody (CL12822)

Images

 
Western Blot: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Analysis in human cell line U-2 OS.
Immunocytochemistry/ Immunofluorescence: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of U2-OS cells using the Anti-TFE3 monoclonal antibody, showing specific staining in nucleoplasm in green. ...read more
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human kidney cancer shows strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human ovarian cancer (clear cell carcinoma) shows moderate nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human placenta shows moderate nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (CL12822) [NBP3-18540] - Staining of human kidney shows weak to moderate nuclear positivity in cells in tubules.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
CL12822
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Transcription factor E3 Antibody (CL12822) Summary

Immunogen
This antibody was developed against a recombinant protein of P19532. Actual immunogen sequence is proprietary.
Predicted Species
Mouse (93%), Rat (92%). Backed by our 100% Guarantee.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TFE3
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 -1:2500
  • Western Blot 1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for Transcription factor E3 Antibody (CL12822)

  • BHLHE33
  • bHLHe33transcription factor E3
  • Class E basic helix-loop-helix protein 33
  • RCCP2
  • TFEA
  • transcription factor binding to IGHM enhancer 3
  • transcription factor E family, member A
  • Transcription factor for IgH enhancer

Background

TFE3, a member of the helix-loop-helix family of transcription factors, binds to the mu-E3 motif of the immunoglobulin heavy-chain enhancer and is expressed in many cell types (Henthorn et al., 1991 [PubMed 1685140]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Transcription factor E3 Antibody (NBP3-18540) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TFE3