Reactivity | HuSpecies Glossary |
Applications | PAGE, Bioactivity |
Format | Carrier-Free |
Concentration | LYOPH |
Description | A single non-glycosylated polypeptide chain containing 132 amino acids corresponding to TRAILR2/TNFRSF10B Source: E. coli Uniprot ID: O14763 Amino Acid Sequence: ESALITQQDL APQQRAAPQQ KRSSPSEGLC PPGHHISEDG RDCISCKYGQ DYSTHWNDLL FCLRCTRCDS GEVELSPCTT TRNTVCQCEE GTFREEDSPE MCRKCRTGCP RGMVKVGDCT PWSDIECVHK ES |
Details of Functionality | TRAIL-R2 protein is fully biologically active when compared to standard. rHusTRAIL-R2 reduced the production of LPS-induced TNF by its ability to neutralize endogenous TRAIL in fresh human PBMC. In this assay, endogenous TRAIL is induced during a 24 hour exposure to LPS (10 ng/mL) but in the presence of rHusTRAIL-R2, TRAIL-induced TNF is suppressed. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | TNFRSF10B |
Purity | > 97 % pure by SDS-PAGE and HPLC |
Endotoxin Note | Less than 1 EU/ug of TRAILR2/TNFRSF10B as determined by LAL method. |
Dilutions |
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Theoretical MW | 14.8 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | > 97 % pure by SDS-PAGE and HPLC |
Reconstitution Instructions | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
Research Areas for TRAILR2/TNFRSF10B Recombinant Protein (NBP2-35030)Find related products by research area.
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