I need to know about TNF-alpha antibody kits for mice.

We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.

I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.

I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.

I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?

The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.

Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?

I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

TNF-alpha Antibody (MP6-XT22) [Unconjugated] (MAB4101): Novus Biologicals

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	MuSpecies Glossary
Applications	B/N, Flow
Clone	MP6-XT22
Clonality	Monoclonal
Host	Rat
Conjugate	Unconjugated
Concentration	LYOPH

Catalog# & Conjugate	Size	Price
Alexa Fluor 350 IC4101U-100UG

Alexa Fluor 405 IC4101V-100UG

Alexa Fluor 488 IC4101G-100UG

Alexa Fluor 532 FAB4101X

Alexa Fluor 594 IC4101T-100UG

Alexa Fluor 647 IC4101R-100UG

Alexa Fluor 700 IC4101N-100UG

Alexa Fluor 750 IC4101S-100UG

Allophycocyanin FAB4101A

Biotin FAB4101B

CoraFluor 1 FAB4101CL1

DyLight 350 FAB4101D

DyLight 405 FAB4101E

DyLight 488 FAB4101K

DyLight 550 FAB4101L

DyLight 594 FAB4101M

DyLight 650 FAB4101W

DyLight 680 FAB4101Y

DyLight 755 FAB4101Z

Fluorescein IC410F

HRP FAB4101H

Janelia Fluor 525 FAB4101JF525

Janelia Fluor 549 FAB4101I

Janelia Fluor 646 FAB4101J

Janelia Fluor 669 FAB4101JF669

PerCP FAB4101C

Phycoerythrin IC410P

mFluor Violet 450 SE FAB4101MFV450

mFluor Violet 500 SE FAB4101MFV500

mFluor Violet 610 SE FAB4101MFV610

Catalog# & Formulation	Size	Price
Carrier Free IC410F

Carrier Free IC410P

Carrier Free MAB4101-SP

Carrier Free IC4101T-100UG

Carrier Free IC4101R-100UG

Carrier Free IC4101G-100UG

Carrier Free IC4101N-100UG

Carrier Free IC4101V-100UG

Carrier Free IC4101S-100UG

Carrier Free IC4101U-100UG

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC4101U-100UG
Alexa Fluor 405	IC4101V-100UG
Alexa Fluor 488	IC4101G-100UG
Alexa Fluor 532	FAB4101X
Alexa Fluor 594	IC4101T-100UG
Alexa Fluor 647	IC4101R-100UG
Alexa Fluor 700	IC4101N-100UG
Alexa Fluor 750	IC4101S-100UG
Allophycocyanin	FAB4101A
Biotin	FAB4101B
CoraFluor 1	FAB4101CL1
DyLight 350	FAB4101D
DyLight 405	FAB4101E
DyLight 488	FAB4101K
DyLight 550	FAB4101L
DyLight 594	FAB4101M
DyLight 650	FAB4101W
DyLight 680	FAB4101Y
DyLight 755	FAB4101Z
Fluorescein	IC410F
HRP	FAB4101H
Janelia Fluor 525	FAB4101JF525
Janelia Fluor 549	FAB4101I
Janelia Fluor 646	FAB4101J
Janelia Fluor 669	FAB4101JF669
PerCP	FAB4101C
Phycoerythrin	IC410P
mFluor Violet 450 SE	FAB4101MFV450
mFluor Violet 500 SE	FAB4101MFV500
mFluor Violet 610 SE	FAB4101MFV610

TNF-alpha Antibody (MP6-XT22) [Unconjugated]

Species Glossary

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TNF-alpha Antibody (MP6-XT22) [Unconjugated] Summary

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Notes

Alternate Names for TNF-alpha Antibody (MP6-XT22) [Unconjugated]

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Publications for TNF-alpha Antibody (MAB4101)(35)

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FAQs for TNF-alpha Antibody (MAB4101). (Showing 1 - 4 of 4 FAQs).

Secondary Antibodies

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Bioinformatics

Immunogen	mouse TNF-alpha
Specificity	Detects mouse TNF-alpha .
Source	N/A
Isotype	IgG1
Clonality	Monoclonal
Host	Rat
Gene	TNF
Purity Statement	Protein A or G purified from hybridoma culture supernatant
Endotoxin Note	<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative	No Preservative
Concentration	LYOPH
Reconstitution Instructions	Reconstitute at 0.5 mg/mL in sterile PBS.

Publications using MAB4101	Applications	Species
Yee Mon, KJ;Kim, S;Dai, Z;West, JD;Zhu, H;Jain, R;Grimson, A;Rudd, BD;Singh, A; Functionalized nanowires for miRNA-mediated therapeutic programming of naïve T cells Nature nanotechnology 2024-04-29 [PMID: 38684809] (Flow Cytometry, Transgenic Mouse)	Flow Cytometry	Transgenic Mouse
Oh, J;Riek, AE;Bauerle, KT;Dusso, A;McNerney, KP;Barve, RA;Darwech, I;Sprague, JE;Moynihan, C;Zhang, RM;Kutz, G;Wang, T;Xing, X;Li, D;Mrad, M;Wigge, NM;Castelblanco, E;Collin, A;Bambouskova, M;Head, RD;Sands, MS;Bernal-Mizrachi, C; Embryonic vitamin D deficiency programs hematopoietic stem cells to induce type 2 diabetes Nature communications 2023-06-13 [PMID: 37311757] (Neutralization, Mouse)	Neutralization	Mouse
Y Li, Y Zhu, S Feng, Y Ishida, TP Chiu, T Saito, S Wang, DK Ann, JJ Ou Macrophages activated by hepatitis B virus have distinct metabolic profiles and suppress the virus via IL-1beta to downregulate PPARalpha and FOXO3 Cell Reports, 2022-01-25;38(4):110284. 1/25/2022 [PMID: 35081341]

H Zhang, G Chen, X Feng, H Song, L Meng, Y Fu, J Yang, Z Fan, Y Ding, Z Du, J Wang, L Yang, J Zhang, L Sun, Z Liu, Z Zhang, Q Li, X Fan Targeting WDxR motif reprograms immune microenvironment and inhibits hepatocellular carcinoma progression Embo Molecular Medicine, 2023-03-22;0(0):e15924. 3/22/2023 [PMID: 36947051]
André C. Ferreira, Carolina Q. Sacramento, Filipe S. Pereira-Dutra, Natália Fintelman-Rodrigues, Priscila P. Silva, Mayara Mattos et al. Severe influenza infection is associated with inflammatory programmed cell death in infected macrophages Frontiers in Cellular and Infection Microbiology 2023-02-16 [PMID: 36875528]
Jian Wu, Feng-Ling Luo, Yan Xie, Huan Xiong, Yadong Gao, Guanghui Liu et al. EST12 regulates Myc expression and enhances anti-mycobacterial inflammatory response via RACK1-JNK-AP1-Myc immune pathway Frontiers in Immunology 2022-08-08 [PMID: 36003390]
Lijuan Sun, Tim Kees, Ana Santos Almeida, Bodu Liu, Xue-Yan He, David Ng et al. Activating a collaborative innate-adaptive immune response to control metastasis Cancer Cell 2021-10-01 [PMID: 34478639]
Vishal Kothari, Jingjing Tang, Yi He, Farah Kramer, Jenny E. Kanter, Karin E. Bornfeldt ADAM17 Boosts Cholesterol Efflux and Downstream Effects of High-Density Lipoprotein on Inflammatory Pathways in Macrophages Arteriosclerosis, Thrombosis, and Vascular Biology 2021-06-01 [PMID: 33882688]
J Cai, D Hu, J Sakya, T Sun, D Wang, L Wang, X Mao, Z Su ABIN-1 is a key regulator in RIPK1-dependent apoptosis (RDA) and necroptosis, and ABIN-1 deficiency potentiates necroptosis-based cancer therapy in colorectal cancer Cell Death & Disease, 2021-02-01;12(2):140. 2021-02-01 [PMID: 33542218] (IP, Mouse)	IP	Mouse
Zhijun Liu, Himani Nailwal, Jonah Rector, Masmudur M. Rahman, Richard Sam, Grant McFadden et al. A class of viral inducer of degradation of the necroptosis adaptor RIPK3 regulates virus-induced inflammation Immunity 2021-02-01 [PMID: 33444549]
Charlie H.T. Kwok, Annastazia E Learoyd, Julia Canet-Pons, Tuan Trang, Maria Fitzgerald Spinal interleukin-6 contributes to central sensitisation and persistent pain hypersensitivity in a model of juvenile idiopathic arthritis Brain, Behavior, and Immunity 2020-11-01 [PMID: 32791212]
Emma V Morris, Karla J Suchacki, Joseph Hocking, Rachel Cartwright, Aneka Sowman, Beatriz Gamez et al. Myeloma Cells Down‐Regulate Adiponectin in Bone Marrow Adipocytes Via TNF‐Alpha Journal of Bone and Mineral Research 2020-05-01 [PMID: 31886918]
L Shi, J Wang, N Ding, Y Zhang, Y Zhu, S Dong, X Wang, C Peng, C Zhou, L Zhou, X Li, H Shi, W Wu, X Long, C Wu, W Liao Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy Nat Commun, 2019-11-28;10(1):5421. 2019-11-28 [PMID: 31780645] (Cell Culture, Mouse)	Cell Culture	Mouse
GJ Song, MH Rahman, MK Jha, DP Gupta, SH Park, JH Kim, SH Lee, IK Lee, T Sim, YC Bae, WH Lee, K Suk A Bcr-Abl Inhibitor GNF-2 Attenuates Inflammatory Activation of Glia and Chronic Pain Front Pharmacol, 2019-05-20;10(0):543. 2019-05-20 [PMID: 31164822] (ELISA Capture, Mouse)	ELISA Capture	Mouse
Ghazaleh M. Shahriary, Hardeep Kataria, Soheila Karimi-Abdolrezaee Neuregulin-1 Fosters Supportive Interactions between Microglia and Neural Stem/Progenitor Cells Stem Cells International 2019-04-07 [PMID: 31089334]
P Handa, S Thomas, V Morgan-Ste, BD Maliken, E Gochanour, S Boukhar, MM Yeh, KV Kowdley Iron alters macrophage polarization status and leads to steatohepatitis and fibrogenesis J. Leukoc. Biol., 2019-03-05;0(0):. 2019-03-05 [PMID: 30835899] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
B John, C Naczki, C Patel, A Ghoneum, S Qasem, Z Salih, N Said Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC Oncogene, 2019-02-14;0(0):. 2019-02-14 [PMID: 30765860] (Neutralization, Mouse)	Neutralization	Mouse
A Chhana, B Pool, KE Callon, ML Tay, D Musson, D Naot, G McCarthy, S McGlashan, J Cornish, N Dalbeth Monosodium urate crystals reduce osteocyte viability and indirectly promote a shift in osteocyte function towards a proinflammatory and proresorptive state Arthritis Res. Ther., 2018-09-10;20(1):208. 2018-09-10 [PMID: 30201038] (Neutralization, Mouse)	Neutralization	Mouse
DT Quiroga, MC Muñoz, C Gil, M Pffeifer, JE Toblli, UM Steckeling, JF Giani, FP Dominici Chronic administration of the angiotensin type 2 receptor agonist C21 improves insulin sensitivity in C57BL/6 mice Physiol Rep, 2018-08-01;6(16):e13824. 2018-08-01 [PMID: 30156060] (IHC, Western Blot, Mouse)	IHC, Western Blot	Mouse
AD Cook, MC Lee, R Saleh, HW Khiew, AD Christense, A Achuthan, AJ Fleetwood, DC Lacey, JE Smith, I Förster, JA Hamilton TNF and granulocyte macrophage-colony stimulating factor interdependence mediates inflammation via CCL17 JCI Insight, 2018-03-22;3(6):. 2018-03-22 [PMID: 29563337] (Neutralization, Mouse)	Neutralization	Mouse
Yina Chen, Qian Zhao, Yangjie Sun, Yin Jin, Jie Zhang, Jiansheng Wu Melatonin induces anti-inflammatory effects via endoplasmic reticulum stress in RAW264.7 macrophages Molecular Medicine Reports 2018-02-15 [PMID: 29484392]
Raquel Tavares, Sushil Kumar Pathak Helicobacter pylori Secreted Protein HP1286 Triggers Apoptosis in Macrophages via TNF-Independent and ERK MAPK-Dependent Pathways Frontiers in Cellular and Infection Microbiology 2017-02-28 [PMID: 28293545]
SN Schock, NV Chandra, Y Sun, T Irie, Y Kitagawa, B Gotoh, L Coscoy, A Winoto Induction of necroptotic cell death by viral activation of the RIG-I or STING pathway Cell Death Differ, 2017-01-06;0(0):. 2017-01-06 [PMID: 28060376] (Bioassay, Mouse)	Bioassay	Mouse
Presentation of Cryptic Peptides by MHC Class I Is Enhanced by Inflammatory Stimuli J Immunol, 2016-09-19;0(0):. 2016-09-19 [PMID: 27647836] (Neutralization, Mouse)	Neutralization	Mouse
Dankbar B, Fennen M, Brunert D, Hayer S, Frank S, Wehmeyer C, Beckmann D, Paruzel P, Bertrand J, Redlich K, Koers-Wunrau C, Stratis A, Korb-Pap A, Pap T Myostatin is a direct regulator of osteoclast differentiation and its inhibition reduces inflammatory joint destruction in mice. Nat Med, 2015-08-03;21(9):1085-90. 2015-08-03 [PMID: 26236992] (Neutralization, Mouse)	Neutralization	Mouse
Ana J. Chucair-Elliott, Christopher Conrady, Min Zheng, Chandra M. Kroll, Thomas E. Lane, Daniel J. J. Carr Microglia-induced IL-6 protects against neuronal loss following HSV-1 infection of neural progenitor cells Glia 2014-09-01 [PMID: 24807365]
Berod L, Stuve P, Swallow M, Arnold-Schrauf C, Kruse F, Gentilini M, Freitag J, Holzmann B, Sparwasser T MyD88 signalling in myeloid cells is sufficient to prevent chronic mycobacterial infection. Eur J Immunol, 2014-02-13;44(5):1399-409. 2014-02-13 [PMID: 24435955] (Flow Cytometry, Mouse)	Flow Cytometry	Mouse
Ruiz E, Oeztuerk-Winder F, Ventura J A paracrine network regulates the cross-talk between human lung stem cells and the stroma. Nat Commun, 2014-01-01;5(0):3175. 2014-01-01 [PMID: 24430801] (Neutralization, Human)	Neutralization	Human
Bhargava R, Altmann C, Andres-Hernando A, Webb R, Okamura K, Yang Y, Falk S, Schmidt E, Faubel S Acute lung injury and acute kidney injury are established by four hours in experimental sepsis and are improved with pre, but not post, sepsis administration of TNF-alpha antibodies. PLoS ONE, 2013-11-12;8(11):e79037. 2013-11-12 [PMID: 24265742] (Neutralization, Mouse)	Neutralization	Mouse
Sushil Kumar Pathak, Raquel Tavares, Nele de Klerk, Anna-Lena Spetz, Ann-Beth Jonsson Helicobacter pylori Protein JHP0290 Binds to Multiple Cell Types and Induces Macrophage Apoptosis via Tumor Necrosis Factor (TNF)-Dependent and Independent Pathways PLoS ONE 2013-11-01 [PMID: 24223737]
Notomi S, Hisatomi T, Kanemaru T, Takeda A, Ikeda Y, Enaida H, Kroemer G, Ishibashi T Critical involvement of extracellular ATP acting on P2RX7 purinergic receptors in photoreceptor cell death. Am. J. Pathol., 2011-10-08;179(6):2798-809. 2011-10-08 [PMID: 21983632] (Neutralization, Mouse)	Neutralization	Mouse
Gardner TR, Chen Q, Jin Y, Ajuebor MN Toll-like receptor 3 ligand dampens liver inflammation by stimulating Valpha 14 invariant natural killer T cells to negatively regulate gammadeltaT cells. Am. J. Pathol., 2010-02-18;176(4):1779-89. 2010-02-18 [PMID: 20167870] (Neutralization, Mouse)	Neutralization	Mouse
Ismail N, Crossley EC, Stevenson HL, Walker DH Relative importance of T-cell subsets in monocytotropic ehrlichiosis: a novel effector mechanism involved in Ehrlichia-induced immunopathology in murine ehrlichiosis. Infect. Immun., 2007-06-11;75(9):4608-20. 2007-06-11 [PMID: 17562770] (Neutralization, Mouse)	Neutralization	Mouse
Cappello P, Caorsi C, Bosticardo M CCL16/LEC powerfully triggers effector and antigen-presenting functions of macrophages and enhances T cell cytotoxicity. J. Leukoc. Biol., 2003-10-02;75(1):135-42. 2003-10-02 [PMID: 14525962] (Neutralization, Mouse)	Neutralization	Mouse
Show All 35 Publications. Collapse Publications.