![Western Blot: TLE4 Antibody [NBP2-15140] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/TLE4-Antibody-Western-Blot-NBP2-15140-img0003.jpg "Western Blot: TLE4 Antibody [NBP2-15140] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE; antibody diluted at 1:1000.")
| Reactivity | Hu, Rt, Mu, Bv, Ch, XpSpecies Glossary |
| Applications | WB, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human TLE4. The exact sequence is proprietary. |
| Predicted Species | Mouse (100%), Bovine (100%), Xenopus (100%), Chicken (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | TLE4 |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 84 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | 0.1M Tris (pH 7), 0.1M Glycine, 20% Glycerol |
| Preservative | 0.01% Thimerosal |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for TLE4 Antibody (NBP2-15140)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: TLE4 Antibody [NBP2-15140] - DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TLE4 stained by TLE4 antibody [C1C3] diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/TLE4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15140-img0004.jpg "Immunocytochemistry/Immunofluorescence: TLE4 Antibody [NBP2-15140] - DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TLE4 stained by TLE4 antibody [C1C3] diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Paraffin: TLE4 Antibody [NBP2-15140] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/TLE4-Antibody-Immunohistochemistry-Paraffin-NBP2-15140-img0002.jpg "Immunohistochemistry-Paraffin: TLE4 Antibody [NBP2-15140] - Paraffin-embedded SW480 xenograft, using antibody at 1:500 dilution.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")