Home » TIMP-2 » TIMP-2 Antibodies » TIMP-2 Antibody (OTI1A6)
Novus Biologicals products are now on bio-techne.com

TIMP-2 Antibody (OTI1A6)

 (3 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Western Blot: TIMP2 Antibody (1A6) [NBP2-01573] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIMP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TIMP2.
Western Blot: TIMP2 Antibody (1A6) [NBP2-01573] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TIMP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: TIMP2 Antibody (1A6) [NBP2-01573] - Staining of paraffin-embedded Human pancreas tissue using anti-TIMP2 mouse monoclonal antibody.
Flow Cytometry: TIMP2 Antibody (1A6) [NBP2-01573] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TIMP2 antibody, and then analyzed ...read more
Immunohistochemistry-Paraffin: TIMP2 Antibody (1A6) [NBP2-01573] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TIMP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TIMP2 Antibody (1A6) [NBP2-01573] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TIMP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TIMP2 Antibody (1A6) [NBP2-01573] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TIMP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TIMP2 Antibody (1A6) [NBP2-01573] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-TIMP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TIMP2 Antibody (1A6) [NBP2-01573] - Staining of paraffin-embedded Human breast tissue using anti-TIMP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: TIMP2 Antibody (1A6) [NBP2-01573] - Staining of paraffin-embedded Human endometrium tissue using anti-TIMP2 mouse monoclonal antibody.
Flow Cytometry: TIMP2 Antibody (1A6) [NBP2-01573] - Analysis of Jurkat cells, using anti-TIMP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, IHC
Clone
OTI1A6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74521AF350
Alexa Fluor 405
NBP2-74521AF405
Alexa Fluor 488
NBP2-74521AF488
Alexa Fluor 532
NBP2-74521AF532
Alexa Fluor 594
NBP2-74521AF594
Alexa Fluor 647
NBP2-74521AF647
Alexa Fluor 700
NBP2-74521AF700
Alexa Fluor 750
NBP2-74521AF750
Allophycocyanin
NBP2-74521APC
Biotin
NBP2-74521B
CoraFluor 1
NBP2-74521CL1
DyLight 350
NBP2-74521UV
DyLight 405
NBP2-74521V
DyLight 488
NBP2-74521G
DyLight 550
NBP2-74521R
DyLight 594
NBP2-74521DL594
DyLight 650
NBP2-74521C
DyLight 680
NBP2-74521FR
DyLight 755
NBP2-74521IR
FITC
NBP2-74521F
HRP
NBP2-74521H
Janelia Fluor 525
NBP2-74521JF525
Janelia Fluor 549
NBP2-74521JF549
Janelia Fluor 646
NBP2-74521JF646
Janelia Fluor 669
NBP2-74521JF669
PE
NBP2-74521PE
PerCP
NBP2-74521PCP
mFluor Violet 450 SE
NBP2-74521MFV450
mFluor Violet 500 SE
NBP2-74521MFV500
mFluor Violet 610 SE
NBP2-74521MFV610
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-74521
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

TIMP-2 Antibody (OTI1A6) Summary

Immunogen
Full length human recombinant protein of human TIMP2(NP_003246) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TIMP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
Theoretical MW
21.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP2-01573 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TIMP-2 Antibody (OTI1A6)

  • CSC-21Ktissue inhibitor of metalloproteinase 2
  • metalloproteinase inhibitor 2
  • TIMP metallopeptidase inhibitor 2
  • TIMP2
  • TIMP-2
  • Tissue inhibitor of metalloproteinases 2

Background

This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

TIMP-1 [HRP]
DTM100
N/A TIMP-1 [HRP]
N/A TIMP-1 [HRP]
(97 Publications)
Species: Hu
Applications: ELISA
MMP-2 [HRP]
MMP200
N/A MMP-2 [HRP]
N/A MMP-2 [HRP]
(102 Publications)
Species: Ca, Hu, Mu, Po, Rt
Applications: ELISA
MMP-9 [HRP]
DMP900
N/A MMP-9 [HRP]
N/A MMP-9 [HRP]
(220 Publications)
Species: Hu
Applications: ELISA
MMP-14/MT1-MMP
Antibody (128527) [Un ...
MAB9181
Flow Cytometry MMP-14/MT1-MMP Antibody (128527) [Unconjugated]
Immunohistochemistry MMP-14/MT1-MMP Antibody (128527) [Unconjugated]
(11 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, IHC, IP, WB
MMP-3 [HRP]
DMP300
N/A MMP-3 [HRP]
N/A MMP-3 [HRP]
(54 Publications)
Species: Hu
Applications: ELISA
TIMP-3
[Unconjugated]
973-TM
TIMP-3 [Unconjugated]
(27 Publications)
Species: Hu
Applications: InhibAct
MMP-1 Antibody
(36665) [Unconjugated
MAB901
Knockout Validated MMP-1 Antibody (36665) [Unconjugated]
Immunohistochemistry MMP-1 Antibody (36665) [Unconjugated]
Immunohistochemistry MMP-1 Antibody (36665) [Unconjugated]
(40 Publications)
Species: Hu
Applications: IHC, IP, KO, Neut, WB
TNF-alpha
[Unconjugated]
210-TA
SDS-PAGE TNF-alpha [Unconjugated]
Bioactivity TNF-alpha [Unconjugated]
SEC-MALS TNF-alpha [Unconjugated]
(3 Reviews)
(818 Publications)
Species: Hu
Applications: BA
VEGF [HRP]
DVE00
N/A VEGF [HRP]
N/A VEGF [HRP]
N/A VEGF [HRP]
(685 Publications)
Species: Hu
Applications: ELISA
MMP-13 [HRP]
DM1300
N/A MMP-13 [HRP]
N/A MMP-13 [HRP]
(34 Publications)
Species: Hu
Applications: ELISA
MMP-7 [HRP]
DMP700
N/A MMP-7 [HRP]
N/A MMP-7 [HRP]
(39 Publications)
Species: Hu
Applications: ELISA
u-Plasminogen
Activator/Urokinase [ ...
1310-SE
Enzyme Activity u-Plasminogen Activator/Urokinase [Unconjugated]
(14 Publications)
Species: Hu
Applications: EnzAct
IL-6 [HRP]
M6000B
N/A IL-6 [HRP]
N/A IL-6 [HRP]
N/A IL-6 [HRP]
(667 Publications)
Species: Mu
Applications: ELISA
Serpin E1/PAI-1
Antibody - BSA Free
NBP1-19773
Western Blot Serpin E1/PAI-1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence Serpin E1/PAI-1 Antibody - BSA Free
Immunohistochemistry-Paraffin Serpin E1/PAI-1 Antibody - BSA Free
(2 Reviews)
(32 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, WB
MMP-8
908-MP
MMP-8
(10 Publications)
Species: Hu
Applications: EnzAct
TIMP-4 [HRP]
DTM400
N/A TIMP-4 [HRP]
N/A TIMP-4 [HRP]
N/A TIMP-4 [HRP]
(5 Publications)
Species: Hu
Applications: ELISA

Publications for TIMP-2 Antibody (NBP2-01573)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Flow Cytometry, ICC/IF, Immunohistochemistry.


Filter By Application
Flow Cytometry
(1)
ICC/IF
(1)
Immunohistochemistry
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for TIMP-2 Antibody (NBP2-01573) (0)

There are no reviews for TIMP-2 Antibody (NBP2-01573). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TIMP-2 Antibody (NBP2-01573) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional TIMP-2 Products

Research Areas for TIMP-2 Antibody (NBP2-01573)

Find related products by research area.

Blogs on TIMP-2.

Check out the latest blog posts on TIMP-2.
Suppressing breast cancer metastasis: The role of hypoxia-induced RhoB expression and activation
By Jamshed Arslan, Pharm. D., PhD. The Ras homologous (Rho) GTPase family of signaling molecules has over 20 members, which typically cycle between active (GTP-bound) and inactive (GDP-bound) states. These small GTPa...  Read full blog post.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Western blot shows detection of Mouse Anti-Human/Mouse/Rat STAT3 Monoclonal Antibody (MAB1799) using a 1:1000 dilution of HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Goat anti-Mouse IgG Secondary Antibody [HRP]
HAF007
SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.
Mouse IgG1 Isotype Control (MG1)
NBP1-97005-0.5mg

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TIMP-2 Antibody (OTI1A6) and receive a gift card or discount.


Bioinformatics

Gene Symbol TIMP2
Entrez