![Western Blot: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - AsPC-1 whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25719_rabbit-thrombospondin-2-mab-hl1140-azide-and-bsa-free-2120249342756.jpg "Western Blot: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - AsPC-1 whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clone | HL1140 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Thrombospondin-2. The exact sequence is proprietary. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | THBS2 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 130 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Immunocytochemistry Osteopontin/OPN Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteopontin_AF808_Immunocytochemistry__Immunofluorescence_23325.jpg)
![Neutralization Osteopontin/OPN Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteopontin_AF808_Block_Neutralize_8975.jpg)
![Immunohistochemistry Osteopontin/OPN Antibody [Unconjugated]](https://images.novusbio.com/images2/Osteopontin_AF808_Immunohistochemistry_9333.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | THBS2 |
![Immunocytochemistry/Immunofluorescence: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Thrombospondin 2 antibody [HL1140] detects Thrombospondin 2 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25719_rabbit-thrombospondin-2-mab-hl1140-azide-and-bsa-free-2120249355547.jpg "Immunocytochemistry/Immunofluorescence: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Thrombospondin 2 antibody [HL1140] detects Thrombospondin 2 protein at cytoplasm by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:500. Blue: Fluoroshield with DAPI .")
![Immunohistochemistry-Paraffin: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Thrombospondin 2 antibody [HL1140] detects Thrombospondin 2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human pancreatic cancer. Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25719_rabbit-thrombospondin-2-mab-hl1140-azide-and-bsa-free-2120249422662.jpg "Immunohistochemistry-Paraffin: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Thrombospondin 2 antibody [HL1140] detects Thrombospondin 2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human pancreatic cancer. Thrombospondin 2 stained by Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![ELISA: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Sandwich ELISA detection of recombinant human thrombospondin 2 protein using antibodies as below. Capture: Thrombospondin 2 antibody [HL1194] (NBP3-25720) (5 ug/mL) Detection: HRP-conjugated Thrombospondin 2 antibody [HL1140] (NBP3-25719) (1 ug/mL) Please notice that NBP3-25719 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25720. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25719_rabbit-thrombospondin-2-mab-hl1140-azide-and-bsa-free-2120249355595.jpg "ELISA: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Sandwich ELISA detection of recombinant human thrombospondin 2 protein using antibodies as below. Capture: Thrombospondin 2 antibody [HL1194] (NBP3-25720) (5 ug/mL) Detection: HRP-conjugated Thrombospondin 2 antibody [HL1140] (NBP3-25719) (1 ug/mL) Please notice that NBP3-25719 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25720. Please contact us for custom HRP-conjugated antibody.")
![Western Blot: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:300000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25719_rabbit-thrombospondin-2-mab-hl1140-azide-and-bsa-free-212024941598.jpg "Western Blot: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Thrombospondin 2 antibody [HL1140] (NBP3-25719) diluted at 1:300000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![ELISA: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length human thrombospondin 2 protein using antibodies as below. Capture: Thrombospondin 2 antibody [HL1140] (NBP3-25719) (5 ug/mL) Detection: HRP-conjugated Thrombospondin 2 antibody [HL1194] (NBP3-25720) (1 ug/mL) Please notice that NBP3-25720 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25719. Please contact us for custom HRP-conjugated antibody.](http://images.novusbio.com/fullsize/nbp3-25719_rabbit-thrombospondin-2-mab-hl1140-azide-and-bsa-free-2120249413386.jpg "ELISA: Thrombospondin-2 Antibody (HL1140) - Azide and BSA Free [NBP3-25719] - Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length human thrombospondin 2 protein using antibodies as below. Capture: Thrombospondin 2 antibody [HL1140] (NBP3-25719) (5 ug/mL) Detection: HRP-conjugated Thrombospondin 2 antibody [HL1194] (NBP3-25720) (1 ug/mL) Please notice that NBP3-25720 needs to be conjugated to HRP to function as the detection antibody when paired with NBP3-25719. Please contact us for custom HRP-conjugated antibody.")
![N/A Thrombospondin-1 [HRP]](https://images.novusbio.com/images2/Thrombospondin-1_DTSP10_ELISA_199.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_871.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/DATA_VEGF_DVE00_ELISA_872.jpg)
![N/A VEGF [HRP]](https://images.novusbio.com/images2/VEGF_DVE00_ELISA_208.jpg)
![N/A MMP-2 [HRP]](https://images.novusbio.com/images2/DATA_MMP2_MMP200_ELISA_1003.jpg)
![N/A MMP-2 [HRP]](https://images.novusbio.com/images2/MMP2_MMP200_ELISA_474.jpg)
![N/A MMP-9 [HRP]](https://images.novusbio.com/images2/MMP-9_DMP900_ELISA_148.jpg)
![N/A MMP-9 [HRP]](https://images.novusbio.com/images2/DATA_MMP9_DMP900_ELISA_770.jpg)
![Western Blot SPARC Antibody [Unconjugated]](https://images.novusbio.com/images2/SPARC_AF942_Western_Blot_20730.jpg)
![Immunohistochemistry SPARC Antibody [Unconjugated]](https://images.novusbio.com/images2/SPARC_AF942_Immunohistochemistry_10419.jpg)
![Immunocytochemistry SPARC Antibody [Unconjugated]](https://images.novusbio.com/images2/SPARC_AF942_Immunocytochemistry__Immunofluorescence_23321.jpg)
![Simple Western Thrombospondin-4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Thrombospondin4_AF2390_Simple_Western_16991.jpg)
![Western Blot Thrombospondin-4 Antibody [Unconjugated]](https://images.novusbio.com/images2/Thrombospondin-4_AF2390_Western_Blot_11766.jpg)
![Immunocytochemistry/ Immunofluorescence Thrombospondin-4 Antibody [Unconjugated]](https://images.novusbio.com/images/af2390_human-thrombospondin-4-affinity-purified-polyclonal-ab-41202412403583.jpg)
![N/A Angiopoietin-2 [HRP]](https://images.novusbio.com/images2/Angiopoietin-2_DANG20_ELISA_30.jpg)
![N/A Angiopoietin-2 [HRP]](https://images.novusbio.com/images2/DATA_Angiopoietin2_DANG20_ELISA_574.jpg)
![N/A COMP/Thrombospondin-5 [HRP]](https://images.novusbio.com/images2/COMP_DCMP0_ELISA_66.jpg)
![N/A COMP/Thrombospondin-5 [HRP]](https://images.novusbio.com/images2/DATA_COMP_DCMP0_ELISA_632.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")