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Themis Antibody (719945)

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Western blot shows lysates of mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Themis Monoclonal Antibody (Catalog # MAB6816) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for Themis at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Themis Monoclonal Antibody (Catalog # MAB6816) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A ...read more
Mouse thymocytes were stained with Rat Anti-Mouse Themis Monoclonal Antibody (Catalog # MAB6816, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clone
719945
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC6816U-100UG
Alexa Fluor 405
IC6816V-100UG
Alexa Fluor 488
IC6816G-100UG
Alexa Fluor 532
FAB6816X
Alexa Fluor 594
IC6816T-100UG
Alexa Fluor 647
IC6816R-100UG
Alexa Fluor 700
IC6816N-100UG
Alexa Fluor 750
IC6816S-100UG
Biotin
FAB6816B
CoraFluor 1
FAB6816CL1
DyLight 350
FAB6816D
DyLight 405
FAB6816E
DyLight 488
FAB6816K
DyLight 550
FAB6816L
DyLight 594
FAB6816M
DyLight 650
FAB6816W
DyLight 680
FAB6816Y
DyLight 755
FAB6816Z
FITC
FAB6816F
HRP
FAB6816H
Janelia Fluor 525
FAB6816JF525
Janelia Fluor 549
FAB6816I
Janelia Fluor 646
FAB6816J
Janelia Fluor 669
FAB6816JF669
PE
FAB6816P
PerCP
FAB6816C
mFluor Violet 450 SE
FAB6816MFV450
mFluor Violet 500 SE
FAB6816MFV500
mFluor Violet 610 SE
FAB6816MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6816-SP
Carrier Free
IC6816U-100UG
Carrier Free
IC6816T-100UG
Carrier Free
IC6816S-100UG
Carrier Free
IC6816R-100UG
Carrier Free
IC6816N-100UG
Carrier Free
IC6816V-100UG
Carrier Free
IC6816G-100UG
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Themis Antibody (719945) Summary

Immunogen
E. coli-derived recombinant mouse Themis
Lys122-Lys237
Accession # Q8BGW0
Specificity
Detects mouse Themis in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human (rh) Themis (aa 2‑282) is observed under non-reducing conditions, and approximately 10% cross-reactivity with rhThemis (aa 2‑282) is observed under reducing conditions.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
THEMIS
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Themis Antibody (719945)

  • bA325O24.3
  • bA325O24.4
  • C6orf190
  • C6orf207
  • FLJ40584
  • Gasp
  • GRB2-associated protein
  • MGC163388
  • protein THEMIS
  • signaling phosphoprotein specific for T cells
  • SPOT
  • Themis
  • thymocyte selection associated
  • thymocyte selection pathway associated
  • thymocyte-expressed molecule involved in selection
  • TSEPA

Background

Themis (thymocyte-expressed molecule involved in selection), also called Gasp (Grb2-associating protein), is a 72 kDa, 641 amino acid (aa) cytosolic phosphoprotein mainly expressed in late double-negative and double-positive thymocytes. It is involved in thymocyte positive and negative selection through regulation of TCR signaling. Within the region used as an immunogen, mouse Themis shares 87% and 96% aa sequence identity with human and rat Themis, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol THEMIS
Uniprot