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TGF-alpha Antibody (SPM542) [FITC]

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Product Details

Summary
Reactivity Hu, Rb, ZeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, CyTOF-ready
Clone
SPM542
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-34418AF350
Alexa Fluor 532
NBP2-34418AF532
Alexa Fluor 594
NBP2-34418AF594
Alexa Fluor 750
NBP2-34418AF750
DyLight 594
NBP2-34418DL594
Unconjugated
NBP2-34371-0.1mg
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-34371-0.1mg
Azide and BSA Free
NBP2-34418-0.1mg
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TGF-alpha Antibody (SPM542) [FITC] Summary

Immunogen
A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGF-alpha (Uniprot: P01135)
Localization
Cytoplasmic and Extracellular (secreted)
Specificity
This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this monoclonal antibody is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Isotype
IgM Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TGFA
Purity
Protein L or PEG purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein L or PEG purified

Alternate Names for TGF-alpha Antibody (SPM542) [FITC]

  • protransforming growth factor alpha
  • TFGA
  • TGFA
  • TGFalpha
  • TGF-alpha
  • transforming growth factor, alpha
  • transforming growth factor-alpha

Background

Transforming growth factor alpha (TGFalpha) is an acid- and heat-stable 50 amino acid protein originally found in rodents and humans. TGFalpha is 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFalpha binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor beta. TGFalpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFalpha bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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TGF-alpha Antibody
(SPM542) [FITC]
NBP2-34418F
TGF-alpha Antibody (SPM542) [FITC]
Species: Hu, Rb, Ze
Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TGFA