![Western Blot: TGF-alpha Antibody (SPM542) [NBP2-34371] - analysis of lysate from HEK293 cells (30ug) using anti-TGF alpha antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/TGF-alpha-Antibody-SPM542-Western-Blot-NBP2-34371-img0001.jpg "Western Blot: TGF-alpha Antibody (SPM542) [NBP2-34371] - analysis of lysate from HEK293 cells (30ug) using anti-TGF alpha antibody. Image from verified customer review.")
| Reactivity | Hu, Rb, ZeSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | SPM542 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | NBP2-34418AF350 | |||
| Alexa Fluor 532 | NBP2-34418AF532 | |||
| Alexa Fluor 594 | NBP2-34418AF594 | |||
| Alexa Fluor 750 | NBP2-34418AF750 | |||
| DyLight 594 | NBP2-34418DL594 | |||
| FITC | NBP2-34418F | |||
| Description | 200ug/ml of antibody purified from Bioreactor Concentrate. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34418) Antibody with azide - store at 2 to 8C. |
| Immunogen | A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGF-alpha (Uniprot: P01135) |
| Localization | Cytoplasmic and Extracellular (secreted) |
| Specificity | This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this monoclonal antibody is completely blocked by the peptide used for raising this antibody. TGF (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. |
| Isotype | IgM Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TGFA |
| Purity | Protein L or PEG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined. |
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| Theoretical MW | 6 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Reviewed Applications |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein L or PEG purified |
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| Images | Ratings | Applications | Species | Date | Details | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
-(05-ml)_NBP2-34371_11141.jpg)
Enlarge |
reviewed by:
Verified Customer |
WB | Human | 10/10/2014 |
Summary
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Secondary Antibodies |
Isotype Controls |
Research Areas for TGF-alpha Antibody (NBP2-34371)Find related products by research area.
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