![Western Blot: TET2 Antibody (649) [NBP2-43552] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [649] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/TET2-Antibody-649-Western-Blot-NBP2-43552-img0006.jpg "Western Blot: TET2 Antibody (649) [NBP2-43552] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [649] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IP, ChIP |
| Clone | 649 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human TET2. The exact sequence is proprietary. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | TET2 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 224 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TET2 |
![Chromatin Immunoprecipitation: TET2 Antibody (649) [NBP2-43552] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control mouse IgG or anti-TET2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.](http://images.novusbio.com/fullsize/TET2-Antibody-649-Chromatin-Immunoprecipitation-NBP2-43552-img0001.jpg "Chromatin Immunoprecipitation: TET2 Antibody (649) [NBP2-43552] - Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control mouse IgG or anti-TET2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.")
![Immunoprecipitation: TET2 Antibody (649) [NBP2-43552] - TET2 antibody [GT649] immunoprecipitates TET2 protein in IP experiments.IP samples: 30 ug whole cell extract of TET2-transfected 293T cells.A. Control with 5 ug of preimmune Rabbit IgGB. Immunoprecipitation of TET2 protein by 5 ug TET2 antibody [GT649] (NBP2-43552)5 % SDS-PAGEThe immunoprecipitated TET2 protein was detected by TET2 antibody [GT649] (NBP2-43552) diluted at 1:1000.[EasyBlot anti-rabbit IgG was used as a secondary reagent]](http://images.novusbio.com/fullsize/nbp2-43552_mouse-monoclonal-tet2-antibody-649-91020242041263.jpg "Immunoprecipitation: TET2 Antibody (649) [NBP2-43552] - TET2 antibody [GT649] immunoprecipitates TET2 protein in IP experiments.IP samples: 30 ug whole cell extract of TET2-transfected 293T cells.A. Control with 5 ug of preimmune Rabbit IgGB. Immunoprecipitation of TET2 protein by 5 ug TET2 antibody [GT649] (NBP2-43552)5 % SDS-PAGEThe immunoprecipitated TET2 protein was detected by TET2 antibody [GT649] (NBP2-43552) diluted at 1:1000.[EasyBlot anti-rabbit IgG was used as a secondary reagent]")
![Western Blot: TET2 Antibody (649) [NBP2-43552] - Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [GT649] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-43552_mouse-monoclonal-tet2-antibody-649-1610202419405782.jpg "Western Blot: TET2 Antibody (649) [NBP2-43552] - Wild-type (WT) and TET2 knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [GT649] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")