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TDG Antibody (OTI3C11) [CoraFluor™ 1]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI3C11
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-74484AF350
Alexa Fluor 405
NBP2-74484AF405
Alexa Fluor 488
NBP2-74484AF488
Alexa Fluor 532
NBP2-74484AF532
Alexa Fluor 594
NBP2-74484AF594
Alexa Fluor 647
NBP2-74484AF647
Alexa Fluor 700
NBP2-74484AF700
Alexa Fluor 750
NBP2-74484AF750
Biotin
NBP2-74484B
DyLight 350
NBP2-74484UV
DyLight 405
NBP2-74484V
DyLight 488
NBP2-74484G
DyLight 550
NBP2-74484R
DyLight 594
NBP2-74484DL594
DyLight 650
NBP2-74484C
DyLight 680
NBP2-74484FR
DyLight 755
NBP2-74484IR
FITC
NBP2-74484F
HRP
NBP2-74484H
Janelia Fluor 525
NBP2-74484JF525
Janelia Fluor 549
NBP2-74484JF549
Janelia Fluor 646
NBP2-74484JF646
Janelia Fluor 669
NBP2-74484JF669
Unconjugated
NBP2-46091
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-46091
Azide and BSA Free
NBP2-74484
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TDG Antibody (OTI3C11) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Human recombinant protein fragment corresponding to amino acids 160-253 of human TDG(NP_003202) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TDG
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for TDG Antibody (OTI3C11) [CoraFluor™ 1]

  • EC 3.2.2.29
  • TDG
  • thymine-DNA glycosylaseG/T mismatch-specific thymine DNA glycosylase

Background

Thymine DNA glycosylase is encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TDG