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TBK1 Antibody [Allophycocyanin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Allophycocyanin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-97957AF350
Alexa Fluor 405
NBP2-97957AF405
Alexa Fluor 488
NBP2-97957AF488
Alexa Fluor 532
NBP2-97957AF532
Alexa Fluor 594
NBP2-97957AF594
Alexa Fluor 647
NBP2-97957AF647
Alexa Fluor 700
NBP2-97957AF700
Alexa Fluor 750
NBP2-97957AF750
Biotin
NBP2-97957B
CoraFluor 1
NBP2-97957CL1
DyLight 350
NBP2-97957UV
DyLight 405
NBP2-97957V
DyLight 488
NBP2-97957G
DyLight 550
NBP2-97957R
DyLight 594
NBP2-97957DL594
DyLight 650
NBP2-97957C
DyLight 680
NBP2-97957FR
DyLight 755
NBP2-97957IR
FITC
NBP2-97957F
HRP
NBP2-97957H
Janelia Fluor 525
NBP2-97957JF525
Janelia Fluor 549
NBP2-97957JF549
Janelia Fluor 646
NBP2-97957JF646
Janelia Fluor 669
NBP2-97957JF669
PE
NBP2-97957PE
PerCP
NBP2-97957PCP
Unconjugated
NBP2-97957-100ul
mFluor Violet 450 SE
NBP2-97957MFV450
mFluor Violet 500 SE
NBP2-97957MFV500
mFluor Violet 610 SE
NBP2-97957MFV610
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TBK1 Antibody [Allophycocyanin] Summary

Immunogen
Produced in rabbits immunized with E. coli-derived Human TBK1 fragment.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TBK1
Purity
Antigen and protein A Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Antigen and protein A Affinity-purified

Alternate Names for TBK1 Antibody [Allophycocyanin]

  • EC 2.7.11
  • EC 2.7.11.1
  • FLJ11330
  • FTDALS4
  • NAK serine/threonine-protein kinase TBK 1
  • NAK
  • NAKserine/threonine-protein kinase TBK1
  • NF-kappa-B-activating kinase
  • NFKB-Activating Kinase
  • T2K
  • TANK-binding kinase 1NF-kB-activating kinase
  • TBK 1
  • TBK1

Background

NFkappaB-activating kinase (NAK) also known as TANK binding kinase-1 (TBK1) or tumor necrosis factor receptor associated factor 2-associated kinase (T2K) is a serine/threonine protein that belongs to the IkB kinase (IKK) family (1). Similar to IKK alpha and beta (2), NAK induces IkB degradation and NF-kB activity. NAK is activated by phorbol ester tumour promoters and growth factors, whereas catalytically inactive NAK specifically inhibits activation of NF-kB by protein kinase C-epsilon (PKCepsilon). NAK specifically phosphorylates IkB alpha on Serine 36 and NFkB subunit RelA (p65) on Serine 536 (3). NAK is also known to interact with TANK, TRAF-2 and TRIF (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for TBK1 Antibody (NBP2-97957APC). (Showing 1 - 6 of 6 FAQ).

  1. What is the exact sequence this TBK1 antibody recognizes?
    • The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
  2. I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
    • While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
  3. We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
    • Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
  4. Is there a smaller size of your TBK1 antibody?
    • This TBK1 antibody is offered in a smaller 0.025 mg size.
  5. Are there any publications where this TBK1 antibody was used in western blot for mouse?
    • This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
  6. Cant this TBK1 antibody be conjugated to PE for FLOW?
    • Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TBK1