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TBK1 Antibody (108A429) [Janelia Fluor® 585]

 
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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, CaSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IP, KO
Clone
108A429
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 585

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-56705AF350
Alexa Fluor 405
NB100-56705AF405
Alexa Fluor 488
NB100-56705AF488
Alexa Fluor 532
NB100-56705AF532
Alexa Fluor 594
NB100-56705AF594
Alexa Fluor 647
NB100-56705AF647
Alexa Fluor 700
NB100-56705AF700
Alexa Fluor 750
NB100-56705AF750
Biotin
NB100-56705B
CoraFluor 1
NB100-56705CL1
DyLight 350
NB100-56705UV
DyLight 405
NB100-56705V
DyLight 488
NB100-56705G
DyLight 550
NB100-56705R
DyLight 594
NB100-56705DL594
DyLight 650
NB100-56705C
DyLight 680
NB100-56705FR
DyLight 755
NB100-56705IR
FITC
NB100-56705F
HRP
NB100-56705H
Janelia Fluor 525
NB100-56705JF525
Janelia Fluor 549
NB100-56705JF549
Janelia Fluor 635
NB100-56705JF635
Janelia Fluor 646
NB100-56705JF646
Janelia Fluor 669
NB100-56705JF669
Unconjugated
NB100-56705
mFluor Violet 450 SE
NB100-56705MFV450
mFluor Violet 500 SE
NB100-56705MFV500
mFluor Violet 610 SE
NB100-56705MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB100-56705
BSA Free
NB100-56705SS
Azide and BSA Free
NBP2-33243
BSA Free
NB100-56705
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TBK1 Antibody (108A429) [Janelia Fluor® 585] Summary

Immunogen
A synthetic peptide corresponding to amino acids 563-577 (YYQFKKDKAERRLAYC) of human TBK1 was used as immunogen for this antibody (NP_037386).
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TBK1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockout Validated
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for TBK1 Antibody (108A429) [Janelia Fluor® 585]

  • EC 2.7.11
  • EC 2.7.11.1
  • FLJ11330
  • FTDALS4
  • NAK serine/threonine-protein kinase TBK 1
  • NAK
  • NAKserine/threonine-protein kinase TBK1
  • NF-kappa-B-activating kinase
  • NFKB-Activating Kinase
  • T2K
  • TANK-binding kinase 1NF-kB-activating kinase
  • TBK 1
  • TBK1

Background

NFkappaB-activating kinase (NAK) also known as TANK binding kinase-1 (TBK1) or tumor necrosis factor receptor associated factor 2-associated kinase (T2K) is a serine/threonine protein that belongs to the IkB kinase (IKK) family (1). Similar to IKK alpha and beta (2), NAK induces IkB degradation and NF-kB activity. NAK is activated by phorbol ester tumour promoters and growth factors, whereas catalytically inactive NAK specifically inhibits activation of NF-kB by protein kinase C-epsilon (PKCepsilon). NAK specifically phosphorylates IkB alpha on Serine 36 and NFkB subunit RelA (p65) on Serine 536 (3). NAK is also known to interact with TANK, TRAF-2 and TRIF (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TBK1 Antibody (NB100-56705JF585). (Showing 1 - 6 of 6 FAQ).

  1. What is the exact sequence this TBK1 antibody recognizes?
    • The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
  2. I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
    • While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
  3. We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
    • Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
  4. Is there a smaller size of your TBK1 antibody?
    • This TBK1 antibody is offered in a smaller 0.025 mg size.
  5. Are there any publications where this TBK1 antibody was used in western blot for mouse?
    • This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
  6. Cant this TBK1 antibody be conjugated to PE for FLOW?
    • Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

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Isotype Controls

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Bioinformatics

Gene Symbol TBK1