![Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - A. 30 ug PC-12 whole cell lysate/extract 7.5% SDS-PAGE tPA antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-Western-Blot-NBP2-20648-img0019.jpg "Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - A. 30 ug PC-12 whole cell lysate/extract 7.5% SDS-PAGE tPA antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human t-Plasminogen Activator/tPA. The exact sequence is proprietary. |
| Localization | Secreted; extracellular space |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PLAT |
| Purity | Antigen Affinity-purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 63 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7), 20% Glycerol, 1% BSA |
| Preservative | 0.025% Proclin 300 |
| Purity | Antigen Affinity-purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for t-Plasminogen Activator/tPA Antibody (NBP2-20648)Find related products by research area.
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![Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Colon carcinoma. tPA antibody dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-Immunohistochemistry-Paraffin-NBP2-20648-img0021.jpg "Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Colon carcinoma. tPA antibody dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with tPA antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-Western-Blot-NBP2-20648-img0016.jpg "Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Human plasma (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with tPA antibody diluted at 1:2000.")
![Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-Western-Blot-NBP2-20648-img0017.jpg "Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Sample (30 ug of whole cell lysate) A: 293T 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - A. 30 ug Neuro2A whole cell lysate/extract 7.5% SDS-PAGE tPA antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-Western-Blot-NBP2-20648-img0018.jpg "Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - A. 30 ug Neuro2A whole cell lysate/extract 7.5% SDS-PAGE tPA antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Human hepatoma. tPA antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/t-Plasminogen-Activator-tPA-Antibody-Immunohistochemistry-Paraffin-NBP2-20648-img0020.jpg "Immunohistochemistry-Paraffin: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Human hepatoma. tPA antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with t-Plasminogen Activator/tPA antibody (NBP2-20648) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20648_rabbit-polyclonal-t-plasminogen-activator-tpa-antibody-91020242011476.jpg "Western Blot: t-Plasminogen Activator/tPA Antibody [NBP2-20648] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with t-Plasminogen Activator/tPA antibody (NBP2-20648) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")