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SUMO4 Antibody (7X3V7)

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Western Blot: SUMO4 Antibody (7X3V7) [NBP3-16732] - Western blot analysis of extracts of various cell lines, using SUMO4 Rabbit mAb (NBP3-16732) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: SUMO4 Antibody (7X3V7) [NBP3-16732] - Western blot analysis of extracts of various cell lines, using SUMO4 Rabbit mAb (NBP3-16732) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at ...read more
Immunocytochemistry/ Immunofluorescence: SUMO4 Antibody (7X3V7) [NBP3-16732] - Immunofluorescence analysis of U-2 OS cells using SUMO4 Rabbit mAb (NBP3-16732) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear ...read more
Immunohistochemistry-Paraffin: SUMO4 Antibody (7X3V7) [NBP3-16732] - Immunohistochemistry of paraffin-embedded mouse kidney using SUMO4 Rabbit mAb (NBP3-16732) at dilution of 1:100 (40x lens).Perform microwave antigen ...read more
Immunohistochemistry-Paraffin: SUMO4 Antibody (7X3V7) [NBP3-16732] - Immunohistochemistry of paraffin-embedded human colon carcinoma using SUMO4 Rabbit mAb (NBP3-16732) at dilution of 1:100 (40x lens).Perform microwave ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
7X3V7
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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SUMO4 Antibody (7X3V7) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human SUMO4 (Q6EEV6). MANEKPTEEVKTENNNHINLKVAGQDGSVVQFKIKRQTPLSKLMKAYCEPRGLSVKQIRFRFGGQPISGTDKPAQLEMEDEDTIDVFQQPTGGVY
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SUMO4
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for SUMO4 Antibody (7X3V7)

  • dJ281H8.4
  • IDDM5
  • small ubiquitin-like modifier 4 protein
  • Small ubiquitin-like protein 4
  • small ubiquitin-related modifier 4
  • SMT3 suppressor of mif two 3 homolog 2
  • SMT3 suppressor of mif two 3 homolog 4 (S. cerevisiae)
  • SMT3 suppressor of mif two 3 homolog 4 (yeast)
  • SMT3H4
  • SUMO4
  • SUMO-4

Background

SUMO4 is a member of the SUMO gene family. This family of genes encode small ubiquitin-related modifiers that are attached to proteins and control the target proteins' subcellular localization, stability, or activity. The protein described in this record is located in the cytoplasm and specifically modifies IKBA, leading to negative regulation of NF-kappa-B-dependent transcription of the IL12B gene. A specific polymorphism in this SUMO gene, which leads to the M55V substitution, has been associated with type I diabetes. The RefSeq contains this polymorphism. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SUMO4