![Western Blot: STK35 Antibody [NB100-1393] - Staining of nuclear HEK293 with antibody at 2 ug/mL (A) and Human Breast cancer with antibody at 1 ug/mL (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/STK35-Antibody-Western-Blot-NB100-1393-img0005.jpg "Western Blot: STK35 Antibody [NB100-1393] - Staining of nuclear HEK293 with antibody at 2 ug/mL (A) and Human Breast cancer with antibody at 1 ug/mL (B) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
| Reactivity | Hu, Mu, Po, Rt, Bv, CaSpecies Glossary |
| Applications | WB, ELISA, Flow, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Goat |
| Conjugate | Unconjugated |
| Concentration | 0.5 mg/ml |
| Immunogen | Peptide with sequence ELETRMDQVTCAA corresponding to C-Terminus according to NP_543026.2. |
| Predicted Species | Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Goat |
| Gene | STK35 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA |
| Preservative | 0.02% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Immunogen affinity purified |
| Publication using NB100-1393 | Applications | Species |
|---|---|---|
| Vallenius T, Makela TP. Clik1: a novel kinase targeted to actin stress fibers by the CLP-36 PDZ-LIM protein. J Cell Sci 2002-05-15 [PMID: 11973348] |
Secondary Antibodies |
Isotype Controls |
Research Areas for STK35 Antibody (NB100-1393)Find related products by research area.
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| Gene Symbol | STK35 |
| Entrez | |
| Uniprot |
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![Immunocytochemistry/Immunofluorescence: STK35 Antibody [NB100-1393] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/STK35-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1393-img0007.jpg "Immunocytochemistry/Immunofluorescence: STK35 Antibody [NB100-1393] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: STK35 Antibody [NB100-1393] - Staining of Human Testis with antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.](http://images.novusbio.com/fullsize/STK35-Antibody-Immunohistochemistry-Paraffin-NB100-1393-img0002.jpg "Immunohistochemistry-Paraffin: STK35 Antibody [NB100-1393] - Staining of Human Testis with antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.")
![Flow Cytometry: STK35 Antibody [NB100-1393] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/STK35-Antibody-Flow-Cytometry-NB100-1393-img0006.jpg "Flow Cytometry: STK35 Antibody [NB100-1393] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")
![Western Blot: STK35 Antibody [NB100-1393] - Staining of Mouse with antibody at 1 ug/mL (A) and Pig with antibody at 2 ug/mL (B). Testes lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/STK35-Antibody-Western-Blot-NB100-1393-img0004.jpg "Western Blot: STK35 Antibody [NB100-1393] - Staining of Mouse with antibody at 1 ug/mL (A) and Pig with antibody at 2 ug/mL (B). Testes lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
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