ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (3) lacks the exon containing the translation start site compared to variant 1, resulting in an isoform (b) with a shorter N-terminus compared to isoform a. Isoform b is likely to be a soluble rather than membrane-bound protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBL1-16496

• ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibodies
• ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Antibody Pair
• ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 ELISA Kit
• ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Lysates
• ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Protein
• ST6 Gal Sialyltransferase 1/ST6GAL1/CD75 Proteins and Enzyme