Home » SS18 » SS18 Lysates » SS18 Overexpression Lysate
Novus Biologicals products are now on bio-techne.com

SS18 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: SS18 Overexpression Lysate (Adult Normal) [NBP2-08819] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SS18.
Western Blot: SS18 Overexpression Lysate (Adult Normal) [NBP2-08819] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SS18.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

SS18 Overexpression Lysate Summary

Description

SS18 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC219498

Accession#: NM_001007559

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
SS18

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
45.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for SS18 Overexpression Lysate

  • MGC116875
  • Protein SYT
  • SSXTsynovial sarcoma, translocated to X chromosome
  • Synovial sarcoma translocated to X chromosome protein
  • synovial sarcoma translocation, chromosome 18
  • SYTprotein SSXT

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

SSX2 Antibody (1A4)
H00006757-M01
Immunohistochemistry-Paraffin SSX2 Antibody (1A4)
Western Blot SSX2 Antibody (1A4)
ELISA SSX2 Antibody (1A4)
(1 Review)
(2 Publications)
Species: Hu
Applications: ELISA, IHC, IHC-P, WB
SSX1 Antibody
(OTI1E10)
NBP2-00614
Flow Cytometry SSX1 Antibody (OTI1E10)
Flow Cytometry SSX1 Antibody (OTI1E10)
Flow Cytometry SSX1 Antibody (OTI1E10)
(1 Publication)
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
P11 Antibody
NBP1-82458
Immunohistochemistry-Paraffin P11 Antibody
Immunohistochemistry-Paraffin P11 Antibody
Immunohistochemistry-Paraffin P11 Antibody
Species: Hu
Applications: IHC, IHC-P
S100A10 Antibody
[Unconjugated]
AF2377
Western Blot S100A10 Antibody [Unconjugated]
Immunohistochemistry S100A10 Antibody [Unconjugated]
Simple Western S100A10 Antibody [Unconjugated]
(22 Publications)
Species: Mu
Applications: IHC, Simple Western, WB
Exosome component
6 Antibody
NBP1-57508
Western Blot Exosome component 6 Antibody
Immunohistochemistry-Paraffin Exosome component 6 Antibody
Immunohistochemistry-Paraffin Exosome component 6 Antibody
Species: Hu
Applications: IHC, IHC-P, WB
Synaptotagmin 1
Antibody (ASV48) [Unc ...
MAB4364
Immunocytochemistry Synaptotagmin 1 Antibody (ASV48) [Unconjugated]
Immunohistochemistry Synaptotagmin 1 Antibody (ASV48) [Unconjugated]
Immunocytochemistry Synaptotagmin 1 Antibody (ASV48) [Unconjugated]
(2 Publications)
Species: Rt
Applications: ICC, IHC, IP, KO, WB
Synaptotagmin 2
Antibody
NB100-91297
Immunohistochemistry Synaptotagmin 2 Antibody
Immunohistochemistry Synaptotagmin 2 Antibody
Immunohistochemistry Synaptotagmin 2 Antibody
Species: Hu, Mu, Pm, Rt
Applications: IHC, IHC-P, WB
Synaptotagmin 4
Antibody (OTI4H3)
NBP2-46045
Immunocytochemistry/ Immunofluorescence Synaptotagmin 4 Antibody (OTI4H3)
Immunocytochemistry/ Immunofluorescence Synaptotagmin 4 Antibody (OTI4H3)
Immunohistochemistry Synaptotagmin 4 Antibody (OTI4H3)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
BRM Antibody
NBP3-13283
Immunohistochemistry-Paraffin BRM Antibody
Immunohistochemistry-Paraffin BRM Antibody
Immunocytochemistry/ Immunofluorescence BRM Antibody
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Brg1 Antibody
[Unconjugated]
AF5738
Western Blot Brg1 Antibody [Unconjugated]
Immunocytochemistry Brg1 Antibody [Unconjugated]
Immunocytochemistry Brg1 Antibody [Unconjugated]
(1 Review)
(3 Publications)
Species: Hu
Applications: ICC, KO, WB
SSX4 Antibody
NBP2-86833
Immunohistochemistry SSX4 Antibody
Western Blot SSX4 Antibody
Western Blot SSX4 Antibody
Species: Hu
Applications: IHC, IHC-P, WB
EWSR1 Antibody
NB200-182
Immunohistochemistry-Paraffin EWSR1 Antibody
Immunoprecipitation EWSR1 Antibody
Western Blot EWSR1 Antibody
(1 Publication)
Species: Hu, Mu
Applications: IHC, IHC-P, IP, Simple Western, WB
Vimentin Antibody
NB300-223
Immunocytochemistry/ Immunofluorescence Vimentin Antibody
Immunohistochemistry-Frozen Vimentin Antibody
Immunocytochemistry/ Immunofluorescence Vimentin Antibody
(6 Reviews)
(70 Publications)
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
CD99 Antibody
[Unconjugated]
AF3968
Flow Cytometry CD99 Antibody [Unconjugated]
Western Blot CD99 Antibody [Unconjugated]
Simple Western CD99 Antibody [Unconjugated]
(4 Publications)
Species: Hu
Applications: CyTOF-ready, Flow, ICC, Simple Western, WB
Bcl-2 Antibody
NB100-56098
Western Blot Bcl-2 Antibody
Western Blot Bcl-2 Antibody
Western Blot Bcl-2 Antibody
(2 Reviews)
(26 Publications)
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, IP, WB
MUC1 Antibody
(SM3) - BSA Free
NB120-22711
Immunocytochemistry/ Immunofluorescence MUC1 Antibody (SM3) - BSA Free
Flow Cytometry MUC1 Antibody (SM3) - BSA Free
Immunocytochemistry/ Immunofluorescence MUC1 Antibody (SM3) - BSA Free
(14 Publications)
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
TRAP220/MED1
Antibody
AF5520
Western Blot TRAP220/MED1 Antibody
(1 Review)
(1 Publication)
Species: Hu, Mu
Applications: WB
FGF acidic/FGF1
Antibody [Unconjugate
AF232
Immunohistochemistry FGF acidic/FGF1 Antibody [Unconjugated]
Simple Western FGF acidic/FGF1 Antibody [Unconjugated]
Western Blot FGF acidic/FGF1 Antibody [Unconjugated]
(13 Publications)
Species: Hu
Applications: IHC, Neut, Simple Western, WB
SMARCA1 Antibody
(2H7B8) - BSA Free
NBP2-61879
ELISA SMARCA1 Antibody (2H7B8) - BSA Free
Flow Cytometry SMARCA1 Antibody (2H7B8) - BSA Free
Immunohistochemistry SMARCA1 Antibody (2H7B8) - BSA Free
Species: Hu, Mu
Applications: ELISA, Flow, IHC, IHC-P, WB
SNAP25 Antibody
(OTI4G5)
NBP2-00795
Flow Cytometry SNAP25 Antibody (OTI4G5)
Western Blot SNAP25 Antibody (OTI4G5)
Immunohistochemistry-Paraffin SNAP25 Antibody (OTI4G5)
(1 Publication)
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB

Publications for SS18 Lysate (NBP2-08819) (0)

There are no publications for SS18 Lysate (NBP2-08819).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for SS18 Lysate (NBP2-08819) (0)

There are no reviews for SS18 Lysate (NBP2-08819). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SS18 Lysate (NBP2-08819) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional SS18 Products

Research Areas for SS18 Lysate (NBP2-08819)

Find related products by research area.

Blogs on SS18

There are no specific blogs for SS18, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Analysis of HeLa cell culture. Antibody at 1:10,000 in green, and costained with mouse mAb to actin, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites.
Vimentin Antibody
NB300-223
Western Blot: Bcl-2 Antibody [NB100-56098] - EA reduced MPP+-induced dopaminergic neuronal apoptosis by increasing BDNF (brain-derived neurotrophic factor) expression and further Akt phosphorylation in the rat substantia nigra. Eight days after MPP+ administration, our Western blot results (MAB7566) show that MPP+ treatment reduced tyrosine hydroxylase and Bcl-2 expression in the ipsilateral side of the rat substantia nigra (SN), but not in the contralateral side. EA stimulation (50 Hz) enhanced mature BDNF, tyrosine hydroxylase, and Bcl-2 expression in the MPP+-treated ipsilateral side. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/18/9/1846), licensed under a CC-BY license.
Bcl-2 Antibody
NB100-56098

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SS18 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol SS18