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SR-BI Antibody [CoraFluor™ 1]

 
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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, ChHa, Ha, Md, Pm, Rb, SyHaSpecies Glossary
Applications WB, Simple Western, Flow, Func, ICC/IF, IHC, IP, B/N, KD, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB400-104AF350
Alexa Fluor 405
NB400-104AF405
Alexa Fluor 488
NB400-104AF488
Alexa Fluor 532
NB400-104AF532
Alexa Fluor 594
NB400-104AF594
Alexa Fluor 647
NB400-104AF647
Alexa Fluor 700
NB400-104AF700
Alexa Fluor 750
NB400-104AF750
Allophycocyanin
NB400-104APC
Biotin
NB400-104B
DyLight 350
NB400-104UV
DyLight 405
NB400-104V
DyLight 488
NB400-104G
DyLight 550
NB400-104R
DyLight 594
NB400-104DL594
DyLight 650
NB400-104C
DyLight 680
NB400-104FR
DyLight 755
NB400-104IR
FITC
NB400-104F
HRP
NB400-104H
Janelia Fluor 525
NB400-104JF525
Janelia Fluor 549
NB400-104JF549
Janelia Fluor 585
NB400-104JF585
Janelia Fluor 635
NB400-104JF635
Janelia Fluor 646
NB400-104JF646
Janelia Fluor 669
NB400-104JF669
PE
NB400-104PE
PerCP
NB400-104PCP
Unconjugated
NB400-104
mFluor Violet 450 SE
NB400-104MFV450
mFluor Violet 500 SE
NB400-104MFV500
mFluor Violet 610 SE
NB400-104MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB400-104
BSA Free
NB400-104SS
BSA Free
NB400-104
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SR-BI Antibody [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
A C-terminal peptide containing residues from mouse SR-BI (within residues 450-509). [UniProt Q61009]
Localization
Cell Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCARB1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Block/Neutralize
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Knockout Validated
  • Proximity Ligation Assay
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:33719499).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Immunogen affinity purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for SR-BI Antibody [CoraFluor™ 1]

  • CD36 and LIMPII analogous 1
  • CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
  • CD36 antigen
  • CD36L1
  • CD36L1scavenger receptor class B type 1
  • CLA1 CD36 antigen-like 1
  • CLA-1 HDLQTL6
  • CLA1
  • Collagen type I receptor, thrombospondin receptor-like 1
  • HDLQTL6
  • MGC138242
  • SCARB1
  • scavenger receptor class B type III
  • scavenger receptor class B, member 1
  • SRB1 scavenger receptor class B member 1
  • SR-B1
  • SRBI
  • SR-BI

Background

SR-BI (scavenger receptor class B member 1, SCARB1) belongs to the CD36 family and acts as a cell surface lipoprotein receptor for a variety of ligands including high density lipoprotein (HDL), phospholipids, phosphatidylserine, and lipoproteins such as lipopolysaccharide (LPS). SR-BI mediates selective uptake of HDL cholesteryl (HDL-C) ester in the liver, steroidogenic tissues, and adipose tissue and facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins. SR-BI plays a role in antigen capture and cross-presentation from bacteria, parasites, and viruses such as Hepatitis C. In endothelial cells and macrophages, SR-BI is involved in anti-inflammatory and pro-survival signaling, providing protection against atherosclerosis and clearing apoptotic cells (efferocytosis) (1, 2).

At least 5 different human splice variants have been identified with theoretical molecular weights ranging from 60.9 kDa for the canonical sequence to 45 kDa for an N-terminally truncated variant (SR-BII) (3). The extracellular domain of human SR-BI (CLA-1) shares 80%, 80%, 89%, 86% and 84% aa sequence identity with mouse, rat, porcine, rabbit, and bovine SR-BI, respectively. SR-BI is highly glycosylated and removal of N-linked glycosylation reduces lipid transport.

References

1. Linton MF, Tao H, Linton EF, Yancey PG. (2017) SR-BI: A Multifunctional Receptor in Cholesterol Homeostasis and Atherosclerosis. Trends Endocrinol Metab. 28(6):461-472. PMID: 28259375

2. Hoekstra M, Sorci-Thomas M. (2017) Rediscovering scavenger receptor type BI: surprising new roles for the HDL receptor. Curr Opin Lipidol. 28(3):255-260. PMID: 28301373

3. Shen WJ, Asthana S, Kraemer FB, Azhar S. (2018) Scavenger receptor B type 1: expression, molecular regulation, and cholesterol transport function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SR-BI Antibody
[CoraFluor™ 1]
NB400-104CL1
SR-BI Antibody [CoraFluor™ 1]
Species: Hu, Mu, Rt, Po, ChHa, Ha, Md, Pm, Rb, SyHa
Applications: WB, Simple Western, Flow, Func, ICC/IF, IHC, IP, B/N, KD, KO

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Product General Protocols

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SCARB1