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SPFH2 Antibody

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Western Blot: SPFH2 Antibody [NB100-1884] - Analysis of SPFH2 on Human Breast lysate using this antibody at 1 ug/ml (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot ...read more
Immunohistochemistry-Paraffin: SPFH2 Antibody [NB100-1884] - staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, ChHaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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SPFH2 Antibody Summary

Immunogen
Peptide with sequence C-EDEPLETATKEN corresponding to C-Terminus according to NP_009106.1.
Specificity
This antibody is expected to recognise isoform 1 (NP_009106) only.
Predicted Species
Rat (100%), Chinese Hamster (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ERLIN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:2000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx. 43 kDa band observed in human breast lysates and in lysates of human breast cancer (PMID: 17502376).
IHC: Paraffin embedded Human Breast.
Control
Human Breast Whole Tissue Lysate (Adult Whole Normal)
Publications
Read Publications using NB100-1884.

Reactivity Notes

Chinese Hamster reactivity reported in scientific literature (PMID: 21343306).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SPFH2 Antibody

  • C8orf2SPFH domain-containing protein 2
  • Endoplasmic reticulum lipid raft-associated protein 2
  • ER lipid raft associated 2
  • Erlin-2
  • MGC87072
  • NET32
  • SPFH domain family, member 2
  • SPFH2chromosome 8 open reading frame 2
  • Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2

Background

SPFH2 is a component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SPFH2 Antibody (NB100-1884)(4)

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Bioinformatics

Gene Symbol ERLIN2
Entrez
Uniprot