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Sp3 Antibody

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Western blot shows nuclear extracts of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and, Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Sp3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4256) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Sp3 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows nuclear extracts of K562 human chronic myelogenous leukemia cell line, HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and, Daudi human Burkitt's lymphoma cell ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
AF4256-SP
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Sp3 Antibody Summary

Immunogen
E. coli-derived recombinant human Sp3
Asp53-Ser309
Accession # Q02447
Specificity
Detects human Sp3 in Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SP3
Purity Statement
Antigen Affinity-purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sp3 Antibody

  • DKFZp686O1631
  • GC-binding transcription factor Sp3
  • Sp3 transcription factor
  • Sp3
  • specificity protein 3
  • SPR2
  • SPR-2
  • transcription factor Sp3

Background

Sp3 is a member of the Sp1 family of C2H2-zinc-finger transcription factors. Sp3 binds to GT and GC boxes in promoters and can either stimulate or repress transcription. Knock out mice indicate that Sp3 is important particularly in erythroid and myeloid cell lineages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SP3
Uniprot