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Smoothelin Antibody (885424) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, CyTOF-ready, Flow
Clone
885424
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC8278U-100UG
Alexa Fluor 405
IC8278V-100UG
Alexa Fluor 488
IC8278G-100UG
Alexa Fluor 532
FAB8278X
Alexa Fluor 594
IC8278T-100UG
Alexa Fluor 647
IC8278R-100UG
Alexa Fluor 700
IC8278N-100UG
Alexa Fluor 750
IC8278S-100UG
Allophycocyanin
FAB8278A
Biotin
FAB8278B
DyLight 350
FAB8278D
DyLight 405
FAB8278E
DyLight 488
FAB8278K
DyLight 550
FAB8278L
DyLight 594
FAB8278M
DyLight 650
FAB8278W
DyLight 680
FAB8278Y
DyLight 755
FAB8278Z
FITC
FAB8278F
HRP
FAB8278H
Janelia Fluor 525
FAB8278JF525
Janelia Fluor 549
FAB8278I
Janelia Fluor 646
FAB8278J
Janelia Fluor 669
FAB8278JF669
PE
FAB8278P
PerCP
FAB8278C
Unconjugated
MAB8278
mFluor Violet 450 SE
FAB8278MFV450
mFluor Violet 500 SE
FAB8278MFV500
mFluor Violet 610 SE
FAB8278MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB8278
Carrier Free
MAB8278
Carrier Free
MAB8278-SP
Carrier Free
IC8278S-100UG
Carrier Free
IC8278R-100UG
Carrier Free
IC8278N-100UG
Carrier Free
IC8278G-100UG
Carrier Free
IC8278V-100UG
Carrier Free
IC8278U-100UG
Carrier Free
IC8278T-100UG
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Smoothelin Antibody (885424) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
E. coli-derived recombinant human Smoothelin B
Met1-Leu132
Accession # P53814
Specificity
Detects human Smoothelin B in direct ELISAs.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SMTN
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunohistochemistry
  • Intracellular Staining by Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Smoothelin Antibody (885424) [CoraFluor™ 1]

  • FLJ35365
  • FLJ38597
  • Smoothelin
  • SMSMO
  • SMTN

Background

Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are a useful tool to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses reveal a broad species distribution of this protein. Cells with SMC like characteristics, such as myofibroblasts, myoepithelial cells, and skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections, as well as transfection studies, show a filamentous organization of smoothelin that is different from that of desmin and vimentin. Smoothelin colocalizes with actin stress fibers. Two tissue specific isoforms have been identified: a 59 kDa isoform specific for visceral SMC; and a 100 kDa specific for vascular SMC. Human smoothelin is encoded by a single copy gene which is located on chromosome 22. The distribution of smoothelin positive cells indicates a correlation between smoothelin expression and smooth muscle tissue contractility. Smoothelin is a useful tool in the evaluation of atherosclerotic lesions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol SMTN