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Human SLC7A5/LAT1 - Ready-To-Use ELISA Kit (Colorimetric)

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ELISA: Human SLC7A5/LAT1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39766] - Standard Curve Reference
ELISA: Human SLC7A5/LAT1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39766] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, cell lysates and other biological fluids
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.056 ng/mL (example only; lot dependent)

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Human SLC7A5/LAT1 - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Standard Curve Range
0.156 - 10 ng/mL (example only; lot dependent)
Sensitivity
0.056 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SLC7A5

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Solution A
  2. Detection Solution B
  3. Instruction manual
  4. Plate sealer for 96 wells
  5. Pre-coated 96T strip plate
  6. Standard Diluent
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human SLC7A5/LAT1 - Ready-To-Use ELISA Kit (Colorimetric)

  • 4F2 LC
  • 4F2LC
  • CD98
  • CD98lc
  • D16S469EL-type amino acid transporter 1
  • E16
  • Integral membrane protein E16
  • large neutral amino acids transporter 1
  • large neutral amino acids transporter small subunit 1
  • LAT1
  • LAT1hLAT1
  • MPE16
  • MPE16CD98 light chain
  • SLC7A5
  • sodium-independent neutral amino acid transporter LAT1,4F2LC
  • solute carrier family 7 (cationic amino acid transporter, y+ system), member 5,4F2 light chain
  • Solute carrier family 7 member 5
  • TA1
  • y+ system cationic amino acid transporter

Background

Mammalian cells have several amino acid transport systems. These systems have been defined by both activity and specific genes. L-type amino acid transporter 1 (LAT1) is a 12 membrane-spanning protein. LAT1 is a Na + -independent neutral amino acid transporter agency and essential for the transporter of large neutral amino acid such as Leucine, Isoleucine, and Valine through the plasma membrane. LAT1 has been proposed to be one of the major nutrient transport systems at the blood-brain barrier. Drugs such as L Dopa are transported by LAT1. LAT1 is thought to be up-regulated to support the high protein synthesis for cell growth in some tumor cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol SLC7A5