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SLC7A5/LAT1 Antibody (EP3-1) [DyLight 350] - Chimeric

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, Func, ICC/IF, IP
Clone
EP3-1
Clonality
Monoclonal
Host
Rabbit
Conjugate
DyLight 350

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP3-20116AF350
Alexa Fluor 405
NBP3-20116AF405
Alexa Fluor 488
NBP3-20116AF488
Alexa Fluor 532
NBP3-20116AF532
Alexa Fluor 594
NBP3-20116AF594
Alexa Fluor 647
NBP3-20116AF647
Alexa Fluor 700
NBP3-20116AF700
Alexa Fluor 750
NBP3-20116AF750
Biotin
NBP3-20116B
CoraFluor 1
NBP3-20116CL1
DyLight 405
NBP3-20116V
DyLight 488
NBP3-20116G
DyLight 550
NBP3-20116R
DyLight 594
NBP3-20116DL594
DyLight 650
NBP3-20116C
DyLight 680
NBP3-20116FR
DyLight 755
NBP3-20116IR
FITC
NBP3-20116F
HRP
NBP3-20116H
Janelia Fluor 525
NBP3-20116JF525
Janelia Fluor 549
NBP3-20116JF549
Janelia Fluor 585
NBP3-20116JF585
Janelia Fluor 635
NBP3-20116JF635
Janelia Fluor 646
NBP3-20116JF646
Janelia Fluor 669
NBP3-20116JF669
PerCP
NBP3-20116PCP
Unconjugated
NBP3-20116-0.2mg
mFluor Violet 450 SE
NBP3-20116MFV450
mFluor Violet 500 SE
NBP3-20116MFV500
mFluor Violet 610 SE
NBP3-20116MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP3-20116-0.2mg
Azide and BSA Free
NBP3-20116-0.2mg
Azide and BSA Free
NBP3-20116-0.025mg
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SLC7A5/LAT1 Antibody (EP3-1) [DyLight 350] - Chimeric Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Immunised mice and constructed IgG1 Fab phage display libraries from the spleen cells. SLC7A5/LAT1-reactive recombinant Fab fragments were retrieved by epitope peptide panning.
Epitope
Shown to bind to epitopes of sequences; LLHGDFHAFSAG-GGGC-NH2, LLHGDFH-GGGC-HN2, and FHAFSAG-GGGC-NH2.
Specificity
Shows no reactivity to live HeLa cells by ICC/IF.
Isotype
IgG Kappa
Clonality
Monoclonal
Host
Rabbit
Gene
SLC7A5
Purity
Protein A purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Functional
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for SLC7A5/LAT1 Antibody (EP3-1) [DyLight 350] - Chimeric

  • 4F2 LC
  • 4F2LC
  • CD98
  • CD98lc
  • D16S469EL-type amino acid transporter 1
  • E16
  • Integral membrane protein E16
  • large neutral amino acids transporter 1
  • large neutral amino acids transporter small subunit 1
  • LAT1
  • LAT1hLAT1
  • MPE16
  • MPE16CD98 light chain
  • SLC7A5
  • sodium-independent neutral amino acid transporter LAT1,4F2LC
  • solute carrier family 7 (cationic amino acid transporter, y+ system), member 5,4F2 light chain
  • Solute carrier family 7 member 5
  • TA1
  • y+ system cationic amino acid transporter

Background

Mammalian cells have several amino acid transport systems. These systems have been defined by both activity and specific genes. L-type amino acid transporter 1 (LAT1) is a 12 membrane-spanning protein. LAT1 is a Na + -independent neutral amino acid transporter agency and essential for the transporter of large neutral amino acid such as Leucine, Isoleucine, and Valine through the plasma membrane. LAT1 has been proposed to be one of the major nutrient transport systems at the blood-brain barrier. Drugs such as L Dopa are transported by LAT1. LAT1 is thought to be up-regulated to support the high protein synthesis for cell growth in some tumor cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SLC7A5