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Skp2 Antibody (712120) [Unconjugated]

 
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Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Skp2 Monoclonal Antibody (Catalog # MAB6817) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Specific bands were detected for Skp2 at approximately 45 and 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U2OS human osteosarcoma cell line. PVDF membrane ...read more
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cells, loaded at 0.2 mg/mL. A specific band was detected for Skp2 at approximately 49 kDa (as indicated) using 20 µg/mL of Mouse ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western
Clone
712120
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB6817-SP
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Skp2 Antibody (712120) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Skp2
Leu130-Pro350
Accession # Q13309
Specificity
Detects human Skp2 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SKP2
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Skp2 Antibody (712120) [Unconjugated]

  • CDK2/cyclin A-associated protein p45
  • cyclin A/CDK2-associated protein p45
  • Cyclin-A/CDK2-associated protein p45
  • FBL1
  • F-box protein Skp2
  • F-box/LRR-repeat protein 1
  • FBXL1
  • FBXL1MGC1366
  • FLB1
  • p45skp2
  • Skp2
  • S-phase kinase-associated protein 2 (p45)
  • S-phase kinase-associated protein 2

Background

Skp2 (S-phase kinase-associated protein 2) is a 45-49 kDa cyclin A- and CDK2-associated phosphoprotein within the FBXL (F-box/LRR) family of ubiquitin ligases, designated FBXL1. Skp2 contains an F-box domain (amino acids (aa) 94-140) and 8 leucine-rich repeats (LRR) between aa 180 and 403. Isoforms of 424 and 410 aa, designated a and b, respectively, diverge after aa 354. As a component of the SCF (SKP1-Cul1-F-box) complex, Skp2 phosphorylation is required for degradation of CDK inhibitors such as p27/Kip1 and progression from G1 to S phases of the cell cycle. Deregulation in cancer promotes cell cycle progression and cancer growth. Within the region used as an immunogen, human Skp2 shares 84% aa sequence identity with mouse and rat Skp2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SKP2
Uniprot