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SIT1 Antibody (SIT-01) [Janelia Fluor® 669]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IP, CyTOF-ready
Clone
SIT-01
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 669

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB500-486AF350
Alexa Fluor 405
NB500-486AF405
Alexa Fluor 488
NB500-486AF488
Alexa Fluor 532
NB500-486AF532
Alexa Fluor 594
NB500-486AF594
Alexa Fluor 647
NB500-486AF647
Alexa Fluor 700
NB500-486AF700
Alexa Fluor 750
NB500-486AF750
Allophycocyanin
NB500-486APC
Allophycocyanin/Cy7
NB500-486APCCY7
Biotin
NB500-486B
CoraFluor 1
NB500-486CL1
DyLight 350
NB500-486UV
DyLight 405
NB500-486V
DyLight 488
NB500-486G
DyLight 550
NB500-486R
DyLight 594
NB500-486DL594
DyLight 650
NB500-486C
DyLight 680
NB500-486FR
DyLight 755
NB500-486IR
FITC
NB500-486F
HRP
NB500-486H
Janelia Fluor 525
NB500-486JF525
Janelia Fluor 549
NB500-486JF549
Janelia Fluor 646
NB500-486JF646
PE
NB500-486PE
PE/Atto594
NB500-486PEATT594
PE/Cy5.5
NB500-486PECY55
PE/Cy7
NB500-486PECY7
PerCP
NB500-486PCP
Unconjugated
NB500-486
mFluor Violet 450 SE
NB500-486MFV450
mFluor Violet 500 SE
NB500-486MFV500
mFluor Violet 610 SE
NB500-486MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NB500-486
BSA Free
NB500-486
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SIT1 Antibody (SIT-01) [Janelia Fluor® 669] Summary

Immunogen
Bacterially produced recombinant intracellular fragment of human SIT.
Specificity
The antibody SIT-01 reacts with SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SIT1
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Weak cross-reactivity with mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for SIT1 Antibody (SIT-01) [Janelia Fluor® 669]

  • gp30/40
  • MGC125908
  • MGC125909
  • RP11-331F9.5
  • SHP2 interacting transmembrane adaptor
  • SHP2-interacting transmembrane adapter protein
  • SHP2-interacting transmembrane adaptor protein
  • signaling threshold regulating transmembrane adaptor 1
  • signaling threshold-regulating transmembrane adapter 1
  • SIT1
  • SITMGC125910
  • suppression inducing transmembrane adaptor 1
  • Suppression-inducing transmembrane adapter 1

Background

Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells. Involved in positive selection of T-cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SIT1