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SHBG Antibody (336729) [Unconjugated]

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Recombinant human SHBG protein was serially diluted 2-fold and captured by Mouse Anti-Human SHBG Monoclonal Antibody (Catalog # MAB26561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SHBG Monoclonal Antibody (Catalog # MAB26562) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Recombinant human SHBG protein was serially diluted 2-fold and captured by Mouse Anti-Human SHBG Monoclonal Antibody (Catalog # MAB26561) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human SHBG ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Clone
336729
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB26562-SP
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SHBG Antibody (336729) [Unconjugated] Summary

Specificity
Detects human SHBG in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
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Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SHBG Antibody (336729) [Unconjugated]

  • ABP
  • ABPTestosterone-estrogen-binding globulin
  • androgen binding protein
  • MGC126834
  • MGC138391
  • SBP
  • sex hormone-binding globulin
  • Sex steroid-binding protein
  • SHBG
  • TEBG
  • Testis-specific androgen-binding protein
  • testosterone-binding beta-globulin
  • Testosterone-estradiol-binding globulin

Background

Human Sex Hormone Binding Globulin (SHBG), also known as ABP or androgen binding protein, is a variably glycosylated, secreted non-disulfide linked homodimer that belongs to the SHBG family. Members of this small family have tandem repeats of 170 aa long laminin a chain G-like domains. Each SHBG monomer is a 47‑53 kDa, 373 aa glycoprotein that contains one steroid-binding site in its N-terminal G-like domain. Male and female sex hormones are bound with equal affinity. SHBG is synthesized by the liver and circulates in blood; ABP (SHBG in the testis) is synthesized by Sertoli cells and circulates in the male reproductive system. Human SHBG shares approximately 67% aa sequence identity with mouse and rat SHBG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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SHBG Antibody
(336729) [Unconjugate
MAB26562
SHBG Antibody (336729) [Unconjugated]
Species: Hu
Applications: ELISA

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