Immunocytochemistry/ Immunofluorescence: SESN3 Antibody [NBP1-31702] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
Western Blot: SESN3 Antibody [NBP1-31702] - A. 30 ug Rat 2 whole cell lysate/extract 7.5 % SDS-PAGESESN3 antibody dilution: 1:1000
Western Blot: SESN3 Antibody [NBP1-31702] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SESN3 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG ...read more
Western Blot: SESN3 Antibody [NBP1-31702] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SESN3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Orthogonal Strategies: Western Blot: SESN3 Antibody [NBP1-31702] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SESN3 antibody diluted at 1:1000. The ...read more
Immunohistochemistry-Paraffin Reported in scientific literature (PMID: 29966750)
Western Blot 1:500-1:3000
Theoretical MW
57 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified
Alternate Names for SESN3 Antibody
SEST3MGC29667
sestrin 3
sestrin-3
Background
Sestrin 3 (SESN3) is a member of the sestrin family of proteins, which modulate peroxide signaling and antioxident defense. p53, a tumor suppressor, regulates the expression of proteins in this family. SESN3 is a promising target for selectively killing cancer cells containing high levels of AKT, a protein kinase that is hyperactivated in cancer cells. AKT is involved in cell proliferation and protects cells from apoptosis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.
Publications for SESN3 Antibody (NBP1-31702)(1)
We have publications tested in 1 application: IHC-P.
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