SESN3 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: SESN3 Antibody [NBP1-31702] - Paraformaldehyde-fixed A549, using antibody at 1:200 dilution.
Western Blot: SESN3 Antibody [NBP1-31702] - A. 30 ug Rat 2 whole cell lysate/extract 7.5 % SDS-PAGESESN3 antibody dilution: 1:1000
Western Blot: SESN3 Antibody [NBP1-31702] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SESN3 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG ...read more
Western Blot: SESN3 Antibody [NBP1-31702] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SESN3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody ...read more
Orthogonal Strategies: Western Blot: SESN3 Antibody [NBP1-31702] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SESN3 antibody diluted at 1:1000. The ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm, RMSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

SESN3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SESN3. The exact sequence is proprietary.
Predicted Species
Chimpanzee (100%), Rhesus Macaque (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SESN3
Purity
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin Reported in scientific literature (PMID: 29966750)
  • Western Blot 1:500-1:3000
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP1-31702.

Reactivity Notes

Xenopus laevis (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Purity
Antigen Affinity-purified

Alternate Names for SESN3 Antibody

  • SEST3MGC29667
  • sestrin 3
  • sestrin-3

Background

Sestrin 3 (SESN3) is a member of the sestrin family of proteins, which modulate peroxide signaling and antioxident defense. p53, a tumor suppressor, regulates the expression of proteins in this family. SESN3 is a promising target for selectively killing cancer cells containing high levels of AKT, a protein kinase that is hyperactivated in cancer cells. AKT is involved in cell proliferation and protects cells from apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for SESN3 Antibody (NBP1-31702)(1)

We have publications tested in 1 application: IHC-P.


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IHC-P
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SESN3 Antibody (NBP1-31702) (0)

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Secondary Antibodies

 

Isotype Controls

Additional SESN3 Products

Array NBP1-31702

Research Areas for SESN3 Antibody (NBP1-31702)

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Blogs on SESN3

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Bioinformatics

Gene Symbol SESN3