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Serpin E1/PAI-1 Antibody (242816) [Unconjugated]

 (9 Publications)
 
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Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin E1/PAI-1 Monoclonal Antibody (Catalog # MAB1786) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin E1/PAI-1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin E1/PAI-1 Monoclonal Antibody (Catalog # MAB1786) followed by HRP-conjugated ...read more
Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for Serpin E1/PAI‑1 at approximately 54 kDa (as indicated) using 2.5 ...read more
Recombinant Human u‑Plasminogen Activator (uPA)/Urokinase (0.1 µg/mL, Catalog # 1310-SE) activity is measured in the presence of Recombinant Human Serpin E1/PAI-1 (0.25 µg/mL, Catalog # 1786-PI) that has been ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, B/N, ELISA(Cap)
Clone
242816
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB1786-SP
Carrier Free
MAB1786-500
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Serpin E1/PAI-1 Antibody (242816) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin E1/PAI-1
Met1-Pro402
Accession # P05121
Specificity
Detects human Serpin E1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Serpin A1, A3, A4, A8, C1, F1, F2, I1, I2, recombinant mouse Serpin D1 or E2 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SERPINE1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.3 ug/mL
  • Simple Western 2.5 ug/mL
  • Western Blot 1 ug/mL
Application Notes
ELISA Capture: Human Serpin E1/PAI-1 Antibody (Catalog # MAB1786)
ELISA Detection: Human Serpin E1/PAI-1 Biotinylated Antibody (Catalog # BAF1786)
Standard: Recombinant Human Serpin E1/PAI-1 (Catalog # 1786-PI)
Publications
Read Publications using
MAB1786 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin E1/PAI-1 Antibody (242816) [Unconjugated]

  • Endothelial plasminogen activator inhibitor
  • Nexin
  • PAI1
  • PAI-1
  • PAI1PAI-1
  • PAISerpin E1
  • PLANH1
  • PLANH1plasminogen activator inhibitor 1
  • serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1
  • Serpin E1
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 1

Background

As a member of the Serpin superfamily of serine protease inhibitors, Serpin E1/PAI‑1 is the principal inhibitor of urokinase‑type plasminogen activator (uPA) and tissue‑type PA (1, 2). As important regulators of extracellular matrix remodeling, uPA and PAI‑1 play a major role in many processes such as angiogenesis, tumor invasion and obesity (2‑4). For example, uPA and PAI-1 are the only tumor prognostic factors validated at the highest level of evidence with regard to their clinical utility in breast cancer (5). The human PAI-1 is initially synthesized as 402 amino acid precursor with a N‑terminal signal peptide (6, 7). PAI‑1 may exist in one of two possible conformations, designated as active or latent (8). The purified rhPAI‑1 is active against rhuPA. The heterogeneity at the N‑terminus of the purified recombinant human PAI‑1 has been observed before for both the recombinant and native proteins (9).

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Stefansson, S. et al. (2003) Curr. Pharm. Des. 9:1545.
  3. Duffy, M.J. (2002) Clin. Chem. 48:1194.
  4. Juhan-Vague, I. et al. (2003) J. Thromb. Haemost. 1:1575.
  5. Harbeck, N. et al. (2002) Clin. Breast Cancer 3:196.
  6. Pannekoek, H. et al. (1986) EMBO J. 5:2539.
  7. Ginsburg, D. et al. (1986) J. Clin. Invest. 78:1673.
  8. Wang, Z. et al. (1996) Biochemistry 35:16443.
  9. Stromqvist, M. et al. (1994) Protein Expr. Purif. 5:309.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Serpin E1/PAI-1 Antibody (MAB1786)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Cell Lysates, Luminex Development, Neutralization, Western Blot.


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Cell Lysates
(1)
Luminex Development
(1)
Neutralization
(3)
Western Blot
(1)
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Human
(6)
All Species
Showing Publications 1 - 9 of 9.
Publications using MAB1786 Applications Species
Y Zhu, LGPL Prata, EOW Gerdes, JME Netto, T Pirtskhala, N Giorgadze, U Tripathi, CL Inman, KO Johnson, A Xue, AK Palmer, T Chen, K Schaefer, JN Justice, AM Nambiar, N Musi, SB Kritchevsk, J Chen, S Khosla, D Jurk, MJ Schafer, T Tchkonia, JL Kirkland Orally-active, clinically-translatable senolytics restore alpha-Klotho in mice and humans EBioMedicine, 2022-03-13;0(0):103912. 2022-03-13 [PMID: 35292270] (Neutralization, Human) Neutralization Human
Christina D. Camell, Matthew J. Yousefzadeh, Yi Zhu, Larissa G. P. Langhi Prata, Matthew A. Huggins, Mark Pierson et al. Senolytics reduce coronavirus-related mortality in old mice Science 7/16/2021 [PMID: 34103349]
Christina D. Camell, Matthew J. Yousefzadeh, Yi Zhu, Larissa G. P. Langhi Prata, Matthew A. Huggins, Mark Pierson et al. Senolytics reduce coronavirus-related mortality in old mice Science 2021-07-16 [PMID: 34103349] (Neutralization, Human) Neutralization Human
AF Aissa, ABMMK Islam, MM Ariss, CC Go, AE Rader, RD Conrardy, AM Gajda, C Rubio-Pere, K Valyi-Nagy, M Pasquinell, LE Feldman, SJ Green, N Lopez-Biga, MV Frolov, EV Benevolens Single-cell transcriptional changes associated with drug tolerance and response to combination therapies in cancer Nature Communications, 2021-03-12;12(1):1628. 2021-03-12 [PMID: 33712615] (Cell Lysates, Human) Cell Lysates Human
Yannick Nossin, Eric Farrell, Wendy J. L. M. Koevoet, Rodrigo A. Somoza, Arnold I. Caplan, Bent Brachvogel et al. Angiogenic Potential of Tissue Engineered Cartilage From Human Mesenchymal Stem Cells Is Modulated by Indian Hedgehog and Serpin E1 Frontiers in Bioengineering and Biotechnology 4/17/2020 [PMID: 32363188]
CD Wiley, S Liu, C Limbad, AM Zawadzka, J Beck, M Demaria, R Artwood, F Alimirah, JA Lopez-Domi, C Kuehnemann, SR Danielson, N Basisty, HG Kasler, TR Oron, PY Desprez, SD Mooney, BW Gibson, B Schilling, J Campisi, P Kapahi SILAC Analysis Reveals Increased Secretion of Hemostasis-Related Factors by Senescent Cells Cell Rep, 2019-09-24;28(13):3329-3337.e5. 2019-09-24 [PMID: 31553904] (Western Blot, Human) Western Blot Human
Bhavani S. Kowtharapu, Radovan Murín, Anselm G. M. Jünemann, Oliver Stachs Role of Corneal Stromal Cells on Epithelial Cell Function during Wound Healing International Journal of Molecular Sciences 2/4/2018 [PMID: 29401709]
Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E A multiplex immunoassay for human adipokine profiling. Clin. Chem., 2010-06-08;56(0):1320. 2010-06-08 [PMID: 20530731] (Luminex Development, Human) Luminex Development Human
Huang MC, Patel K, Taub DD, Longo DL, Goetzl EJ Human CD4- 8- T cells are a distinctive immunoregulatory subset. FASEB J, 2010-02-12;24(7):2558-66. 2010-02-12 [PMID: 20154266] (Neutralization, Human) Neutralization Human

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Bioinformatics

Gene Symbol SERPINE1
Uniprot