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Serpin A8/Angiotensinogen Antibody (754438)

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Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Serpin A8/Angiotensinogen Monoclonal Antibody (Catalog # MAB6966) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Serpin A8/Angiotensinogen at approximately 52-62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Serpin A8/Angiotensinogen Monoclonal Antibody (Catalog # MAB6966) followed by HRP-conjugated Anti-Rat IgG ...read more
Serpin A8/Angiotensinogen was detected in perfusion fixed frozen sections of mouse intestine using Rat Anti-Mouse Serpin A8/Angiotensinogen Monoclonal Antibody (Catalog # MAB6966) at 5 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clone
754438
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
MAB6966-SP
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Serpin A8/Angiotensinogen Antibody (754438) Summary

Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Serpin A8/Angiotensinogen
Asp25-Val477
Accession # P11859
Specificity
Detects mouse Serpin A8/Angiotensinogen in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Serpin A8/Angiotensinogen, rhSerpin A4, recombinant mouse (rm) Serpin A1, A3N, A11, B8, C1, E1, E2, I1, or rmPEDF is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Rat
Gene
AGT
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.5 ug/mL
Publications
Read Publication using MAB6966.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin A8/Angiotensinogen Antibody (754438)

  • AGT
  • alpha-1 antiproteinase, antitrypsin
  • Angiotensin II
  • angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
  • Angiotensinogen
  • ANHU
  • Aogen
  • FLJ92595
  • FLJ97926
  • PAT
  • pre-angiotensinogen
  • serine (or cysteine) proteinase inhibitor
  • Serpin A8
  • SERPINA8angiotensin I

Background

Serpin A8 (serine proteinase inhibitor-clade A #8; also angiotensinogen/ANGT) is a secreted, 52-62 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors.  It is expressed by neurons and hepatocytes, and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I.  This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II.  Ang II induces vascoconstriction and aldosterone release.  Mature human Serpin A8 is 452 aa in length (aa 34-485).  It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41).  Serpin A8 may circulate in a 200 kDa complex with major basic protein (MBP), or as part of a larger 300 kDa complex with MBP and complement C3dg.  Over aa 34-485, human Serpin A8 shares 61% aa identity with mouse Serpin A8.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Serpin A8/Angiotensinogen Antibody (MAB6966)(1)


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Bioinformatics

Gene Symbol AGT
Uniprot